LC/MS/MS Purchase

LC/MS/MS 购买

• 批准号: 6578412
• 负责人: ROBERT S HODGES
• 金额: $ 28.43万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6578412
• 关键词: biomedical equipment purchase; functional /structural genomics; liquid chromatography mass spectrometry; protein purification; proteomics

DESCRIPTION (provided by applicant): The University of Colorado School of Medicine will purchase a LC/MS/MS instrument with support for the NCRR Shared Instrumentation Grant Program. This instrument is required to meet the growing demand for the separation and identification of peptides and proteins. The increased demand results from expansion of the faculty in several departments in the School, the expansion of proteomics initiatives, and the establishment of a new interdepartmental Program in Biomolecular Structure. The requested instrument will vastly improve the existing resources and will accelerate research in the following systems: Methods development for glycoproteomics; identification of cell surface proteins through selection and structural determination of their tryptic glycopeptides. (B. Bendiak) Structure and mechanism in coordinated gene regulation. (M. Churchill) Development of new methodology for multidimensional liquid chromatographic separation of peptides and proteins for proteomic initiatives. (R.S. Hodges) Studies of yeast transcription involving Pafl/RNA polymerase II complex and mitochondrial RNA polymerase. (J.A. Jaehning) Genome scanning and identification of scaffold associated signaling complexes. (G. Johnson) Monitoring the degradation/chemical modification of recombinant proteins important to the biopharmaceutical industry during processing and storage. (M. Manning) Identification of yeast Cdc7-Dbf4 substrates in DNA repair and mutagenesis. (R. Sclafani) Identification and characterization of proteins interacting with epidermal growth factor receptor during endocytosis in normal and cancer cells. (A. Sorkin) Design of peptide analogs/mimetic of the hormones bradykinin and substance P and cloning of the substance P receptor in rat brain. (J. M. Stewart)

描述（由申请人提供）：科罗拉多大学医学院将在 NCRR 共享仪器资助计划的支持下购买一台 LC/MS/MS 仪器。该仪器需要满足对肽和蛋白质的分离和鉴定不断增长的需求。需求的增加是由于学院多个系师资队伍的扩大、蛋白质组学计划的扩大以及新的生物分子结构跨系项目的建立。所需的仪器将极大地改善现有资源，并将加速以下系统的研究： 糖蛋白质组学方法开发；通过胰蛋白酶糖肽的选择和结构测定来鉴定细胞表面蛋白。 （B·本迪亚克） 协调基因调控的结构和机制。 （丘吉尔先生） 开发用于蛋白质组学研究的肽和蛋白质多维液相色谱分离的新方法。 （R.S.霍奇斯） 涉及 Pafl/RNA 聚合酶 II 复合物和线粒体 RNA 聚合酶的酵母转录研究。 （J.A.杰宁） 基因组扫描和支架相关信号复合物的鉴定。 （G·约翰逊） 监测重组蛋白在加工和储存过程中的降解/化学修饰对生物制药行业很重要。 （M·曼宁） DNA 修复和诱变中酵母 Cdc7-Dbf4 底物的鉴定。 （R·斯克拉法尼） 正常细胞和癌细胞内吞过程中与表皮生长因子受体相互作用的蛋白质的鉴定和表征。 （A·索金） 激素缓激肽和 P 物质的肽类似物/模拟物的设计以及大鼠脑中 P 物质受体的克隆。 （J·M·斯图尔特）