UDE COBRE: PEPTIDE-BASED BIOMATERIALS WITH ENVIRON SENSITIVE MORPHOLOGIES

UDE COBRE：具有环境敏感形态的肽基生物材料

• 批准号: 7960410
• 负责人: JOEL P SCHNEIDER
• 金额: $ 40.18万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960410
• 关键词: Amino Acids; Biocompatible; Biocompatible Materials; Biotechnology; Cell Adhesion; Centers of Research Excellence; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Cues; Diffusion; Fibroblasts; Funding; Grant; Hydrogels; Institution; Ionic Strengths; Light; Microfluidics; Molecular; Molecular Conformation; Molecular Weight; Morphology; Mus; Nature; Nutrient; Peptides; Polymers; Property; Prunella vulgaris; Research; Research Personnel; Resources; Source; System; Temperature; Tissue Engineering; United States National Institutes of Health; base; cell motility; crosslink; design; nanoscale; novel; porous hydrogel; response; scaffold; self assembly; wasting

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project II: Schneider and Pochan Hydrogels, a class of materials integral to biotechnologies such as tissue engineering and microfluidics are classically made from high molecular weight polymers. Ideal hydrogel scaffolds for use in tissue engineering are easily synthesized using non-toxic chemistries, biocompatible, promote cell adhesion/proliferation and are biodegradable. In terms of material properties, their morphology should be porous for cell motility and nutrient/waste diffusion. However, despite their porous nature, these materials must be mechanically rigid. We developed an alternate strategy that employs self-assembling peptides to prepare responsive hydrogels. Preparing materials from peptides is advantageous since peptides are quickly synthesized and novel amino acid residues readily incorporated for tailored functions. Also, since our hydrogels are prepared from a pure self-assembly mechanism, there is no need for exogenous toxic cross linking agents. The strategy employs designed peptides that intramolecularly fold on cue into a b-hairpin conformation that is amenable to self-assembly. Key to this system is that the peptide must be intramolecularly folded before self-assembly leading to hydrogelation can occur. By designing peptides that only fold when desired environmental cues are present, responsive materials may be constructed. Hairpins have been designed to fold in response to pH and ionic strength changes, light, and temperature changes. Hydrogels are porous and well hydrated on the micro- and nano-scale, but are rigid (G'=1600Pa), shear thin and self heals after cessation of shear. Importantly, these hydrogels support the attachment and proliferation of NIH 3T3 fibroblasts (mouse), indicating their possible use as tissue engineering scaffolds.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 项目II：施耐德和波昌 水凝胶是一类与生物技术（例如组织工程和微流体学）不可或缺的材料。 理想的水凝胶支架可用于组织工程，很容易使用无毒的化学物质（生物相容性，促进细胞粘附/增殖）合成，并且可生物降解。 就材料特性而言，它们的形态应该是细胞运动和营养/废物扩散的多孔。 但是，尽管它们的性质多孔，但这些材料必须机械刚性。 我们制定了一种替代策略，该策略采用自组装肽来制备响应性水凝胶。 从肽制备材料是有利的，因为肽是快速合成的，并且很容易掺入用于量身定制功能的新型氨基酸残基。 同样，由于我们的水凝胶是根据纯自组装机制制备的，因此不需要外源性有毒交叉链接剂。 该策略采用设计的肽，分子内将提示折叠成B发型构象，该构型可与自组装相提并论。 该系统的关键是，必须在发生自组装之前将肽内分子折叠。 通过设计只有在需要的环境提示时折叠的肽，就可以构建响应材料。 发夹已被设计为响应pH和离子强度变化，光和温度变化而折叠。 水凝胶在微和纳米尺度上是多孔且水合充足的，但刚性（g'= 1600pa），剪切薄，剪切后自治愈。 重要的是，这些水凝胶支持NIH 3T3成纤维细胞（小鼠）的附着和增殖，表明它们可能用作组织工程支架。