Hydrogels from Designed Peptides

来自设计肽的水凝胶

基本信息

批准号：
7344859
负责人：
JOEL P SCHNEIDER
金额：
$ 27.47万
依托单位：
UNIVERSITY OF DELAWARE
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-02-01 至 2010-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7344859
关键词：
Adhesions Affect Benign Biochemistry Biocompatible Materials Biological Body Temperature Calcium ion Cell Adhesion Cell Line Cells Chemicals Chemistry Collaborations Compatible Cues Culture Media Engineering Epitopes Event Exhibits Fibroblasts Gel Hydrogels In Vitro Individual Light Modeling Molecular Molecular Conformation Morphology Muscle Rigidity Nature Osteoblasts Peptides Porosity Preparation Process Property Research Science Sodium Chloride Solutions Stimulus Supporting Cell System Temperature Tissue Engineering Work base bone chemical synthesis crosslink cytotoxic design in vivo nano novel peptide structure porous hydrogel response scaffold self assembly soft tissue viscoelasticity

Adhesions Affect Benign Biochemistry Biocompatible Materials Biological Body Temperature Calcium ion Cell Adhesion Cell Line Cells Chemicals Chemistry Collaborations Compatible Cues Culture Media Engineering Epitopes Event Exhibits Fibroblasts Gel Hydrogels In Vitro Individual Light Modeling Molecular Molecular Conformation Morphology Muscle Rigidity Nature Osteoblasts Peptides Porosity Preparation Process Property Research Science Sodium Chloride Solutions Stimulus Supporting Cell System Temperature Tissue Engineering Work base bone chemical synthesis crosslink cytotoxic design in vivo nano novel peptide structure porous hydrogel response scaffold self assembly soft tissue viscoelasticity

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项目摘要

DESCRIPTION (provided by applicant): The preparation of hydrogels via peptide self-assembly allows one to define ultimate biomaterial properties by design of individual constituent molecules. Chemical functionality, material morphology, viscoelasticity and processibility can be designed at the molecular level of a self-assembling system. Herein, peptides are designed to intramolecularly fold in response to external stimuli into beta-hairpin conformations that are capable of self-assembling. Folded hairpins assemble into dilute but rigid hydrogels exhibiting porosity on the nano- to -microscale. Triggered folding allows temporal and spatial control of material formation. Gelation will be triggered by various physiologically relevant stimuli; e.g. pH, salt, calcium ions, temperature and light. The chemically benign folding/self-assembly strategy, and the inherent peptidic nature of the resultant hydrogels, provide a potential tissue engineering substrate for fibroblasts and osteoblasts. Peptide design principles leading to rigid, porous hydrogels capable of supporting cell adhesion and proliferation will be determined by directly observing how peptide structure affects the self-assembly process, material morphology, material properties and cytocompatibility. The interdisciplinary "molecular to materials" design and properties characterization of the proposed hydrogelation system will be accomplished via close collaboration between the chemistry/biochemistry and materials science and engineering departments. This research effort will: 1. Gain a fundamental understanding of the folding and self-assembly process leading to hydrogel formation and how molecular design affects material properties. 2. Enhance the processibility of hairpin-based hydrogels by designing active intramolecular folding triggers that allow peptide solutions to undergo hydrogelation on cue. 3. Determine how peptide structure and material properties affect the adhesion and proliferation of model fibroblast and osteoblast cell lines.

描述（由申请人提供）：通过肽自组装制备水凝胶可以通过设计单个成分分子的设计来定义最终的生物材料特性。可以在自组装系统的分子水平上设计化学功能，材料形态，粘弹性和过程。在此，肽被设计为分子内响应于外部刺激构成能够自组装的β发蛋白构象。折叠的发夹组装成稀释的水凝胶，在纳米至微尺度上表现出孔隙率。触发的折叠允许对材料形成的时间和空间控制。凝胶化将由各种与生理相关的刺激触发。例如pH，盐，钙离子，温度和光。化学良性折叠/自组装策略以及所得水凝胶的固有肽性质为成纤维细胞和成骨细胞提供了潜在的组织工程底物。通过直接观察肽结构如何影响自组装过程，材料形态，材料特性和细胞相容性，可以确定能够支撑细胞粘附和增殖的固体水凝胶的肽设计原理。跨学科的“材料分子”设计和属性的特征将通过化学/生物化学与材料科学和工程部门之间的密切合作来实现。这项研究工作将：1。对折叠和自组装过程的基本了解，导致水凝胶形成以及分子设计如何影响材料特性。 2。通过设计活跃的分子内折叠触发器来增强基于发夹的水凝胶的过程，从而使肽溶液在提示上进行水凝胶。 3。确定肽结构和材料特性如何影响模型成纤维细胞和成骨细胞系的粘附和增殖。