Analytical Ultracentrifuge Purchase

分析超速离心机购买

• 批准号: 7043735
• 负责人: ROBERT S HODGES
• 金额: $ 43.41万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7043735
• 关键词: Coronaviridae; RNA; protein protein interaction; proteins; schools

DESCRIPTION (provided by applicant): The University of Colorado School of Medicine will purchase an analytical ultracentrifugation instrument with support from the NCRR Shared Instrumentation Grant Program. This instrument is required to meet the growing demand for the accurate and rapid determination of size, shape, association and interactions of macromolecules and complexes. The increased demand results from expansion of the faculty in several departments in the School as well as the establishment of the interdepartmental Program in Biomolecular Structure. The requested instrument will greatly improve the existing resources and will accelerate research in a broad range of systems, including:  Analysis of self-association and heteroprotein interactions in the human progesterone receptor (D.L. Bain)  Obtaining a mechanistic understanding of viral DNA packaging (C.E. Catalano).  De novo design of coiled-coils to understand protein folding and stability and investigations of coiled- coils in native proteins, e.g., motor proteins, transporter of ProP and SARS-coronavirus (R.S. Hodges).  Determining functional differences of isoforms of progesterone (K. Horwitz).  Examining folding of biologically important RNA molecules including functional RNAs from poliovirus and related viruses, and studying protein-RNA interactions from foot and mouth disease virus (J.S. Kieft).  Analysis of protein assembly mechanisms and quantification of strength of protein-protein interactions in cellular replicases (C. McHenry). Other areas of current research also include determination of oligomerization and/or protein-protein interactions in a range of receptors (e.g., B cell complement receptor, glycoproteins of coronavirus, ligand binding proteins of estrogen receptor); the role of ETS transcription factors in epithelial cell development and tumorigenesis; subcellular organization of signal transduction pathways; lymphocyte-specific transcription by early B cell factor; storage and intracellular trafficking of lipids; characterization of tissue plasminogen activator protein; characterization of genetically identified telomerase and end-protection complexes in yeast; and molecular mechanisms of pre-mRNA splicing.

描述（由申请人提供）：科罗拉多大学医学院将在 NCRR 共享仪器资助计划的支持下购买一台分析超速离心仪器。该仪器需要满足对大分子和复合物的尺寸、形状、缔合和相互作用的精确和快速测定日益增长的需求。需求的增加是由于学院多个系师资队伍的扩大以及生物分子结构跨系项目的建立。所要求的工具将极大地改善现有资源，并将加速广泛系统的研究，包括： 人孕酮受体的自关联和异蛋白相互作用分析（D.L. Bain） 获得对病毒 DNA 包装机制的理解 (C.E. Catalano)。 从头设计卷曲螺旋以了解蛋白质折叠和稳定性，并研究天然蛋白质中的卷曲螺旋，例如运动蛋白、ProP 转运蛋白和 SARS 冠状病毒 (R.S. Hodges)。确定黄体酮亚型的功能差异（K. Horwitz）。 检查生物学上重要的 RNA 分子的折叠，包括来自脊髓灰质炎病毒和相关病毒的功能性 RNA，并研究来自口蹄疫病毒的蛋白质-RNA 相互作用 (J.S. Kieft)。 细胞复制酶中蛋白质组装机制的分析和蛋白质-蛋白质相互作用强度的量化（C. McHenry）。 当前研究的其他领域还包括测定一系列受体（例如 B 细胞补体受体、冠状病毒糖蛋白、雌激素受体配体结合蛋白）中的寡聚化和/或蛋白质-蛋白质相互作用； ETS转录因子在上皮细胞发育和肿瘤发生中的作用；信号转导途径的亚细胞组织；早期 B 细胞因子的淋巴细胞特异性转录；脂质的储存和细胞内运输；组织纤溶酶原激活蛋白的表征；酵母中遗传鉴定的端粒酶和末端保护复合物的表征；以及前mRNA剪接的分子机制。