Multiple Peptide Synthesizer - An Integrated System

多肽合成器 - 集成系统

• 批准号: 6440960
• 负责人: ALEXANDER none KUROSKY
• 金额: $ 18.38万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440960
• 关键词: biomedical equipment purchase; high performance liquid chromatography; peptide chemical synthesis; protein purification

DESCRIPTION (provided by applicant): Support is requested for an automated solid-phase peptide synthesizer, preparative high-performance liquid chromatography equipment dedicated to peptide purification, and a vacuum concentrator for drying solvents used during synthesize and purification. This integrated system of instruments for producing synthetic peptides will replace aging ten-year-old equipment that is now unsupported by the manufacturer. The selected synthesizer, a Rainin Symphony/Multiplex with the Cascade Combinatorial Reactor, is a multiple peptide synthesizer that can synthesize up to twelve peptides simultaneously and independently, allowing us to better meet increased demands for synthetic peptides for NIH-funded researchers at the University of Texas Medical Branch (UTMB). The beneficiary users of the requested equipment include a multidisciplinary group of fourteen predominantly NIH-supported investigators (9 major and 5 minor users; 22 funded NIB grants totaling $4 million dollars annual direct costs) representing several clinical and basic science departments at UTMB, e.g. Human Biological Chemistry and Genetics, Microbiology and Immunology, Pediatrics, Pathology, Anatomy and Neuroscience, as well as Internal Medicine. Examples of diseases under investigation by these synthetic peptide users include those involving or related to muscular dystrophy, allergy, asthma, cancer, xenobiotics, environmental toxicology, mutagenesis, and gastroenterology. The Peptide Synthesis Core is one of five cores within the UTMB Protein Chemistry Laboratory; the others are the Protein/DNA Sequencing, the Proteomics, the Mass Spectrometry, and the Protein Expression Cores. Together, these core facilities provide a wide range of services for the entire University campus including multiple campus research centers. Acquisition of the requested instrumentation will significantly enhance the ability of NIH-funded investigators to conduct their ongoing hypothesis-driven research and, moreover, will extend their research opportunities in the future to higher levels of productivity and scientific impact.

描述（由申请人提供）：请求支持自动化 固相肽合成仪、制备高性能液体 专用于肽纯化的色谱设备和真空 用于干燥合成和纯化过程中使用的溶剂的浓缩器。这 用于生产合成肽的仪器集成系统将取代 使用十年的老化设备现在不受制造商支持。这 选定的合成器，带有 Cascade 的 Rainin Symphony/Multiplex 组合反应器，是一种多肽合成仪，可以合成 同时独立地合成十二个肽，使我们能够更好地满足 NIH 资助的研究人员对合成肽的需求增加 德克萨斯大学医学分部 (UTMB)。受益用户 所请求的设备包括一个由十四人组成的多学科小组，主要是 NIH 支持的研究人员（9 位主要用户和 5 位次要用户；22 项 NIB 资助 总计 400 万美元的年度直接费用）代表多个临床 以及 UTMB 的基础科学系，例如人类生物化学和 遗传学、微生物学和免疫学、儿科、病理学、解剖学和 神经科学，以及内科。以下疾病的例子 这些合成肽用户的调查包括涉及或 与肌肉营养不良、过敏、哮喘、癌症、异生素有关， 环境毒理学、诱变和胃肠病学。肽 合成核心是 UTMB 蛋白质化学的五个核心之一 实验室;其他是蛋白质/DNA 测序、蛋白质组学、质量 光谱测定和蛋白质表达核心。这些核心设施共同 为整个大学校园提供广泛的服务，包括 多个校园研究中心。获取所需仪器 将显着提高 NIH 资助的研究人员进行研究的能力 他们正在进行的假设驱动的研究，而且还将扩展他们的研究 未来提高生产力水平的研究机会 科学影响。