UTMB Amino Acid Analyzer

UTMB氨基酸分析仪

• 批准号: 6580766
• 负责人: ALEXANDER none KUROSKY
• 金额: $ 12.61万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580766
• 关键词: aminoacid analyzer; biomedical equipment purchase

DESCRIPTION (provided by applicant): Support is requested for a Hitachi Model L-8800 amino acid analyzer to perform a wide spectrum of amino acid analyses for a relatively large group of biomedical researchers at the University of Texas Medical Branch at Galveston (UTMB). The Hitachi analyzer employs a post-column methodology utilizing either colorimetric detection (ninhydrin) or fluorescence detection (omicron-phthaladehyde). Furthermore, the L-8800 can perform a wide variety of analytical methodologies including protein/peptide hydrolyzates, physiological fluids, and hydrolyzates of specialized samples, such as those of fibrous proteins, or proteins that have been chemically or posttranslationally modified. Compositional analysis and precise analysis of protein/peptide concentrations will be among the critically important applications of the Hitachi analyzer, as well as analysis of free amino acids in samples of physiological fluids and tissue extracts. The requested Hitachi analyzer will replace a 15-year old retired Beckman 6300 and an aging 13-year old Applied Biosystems 420H Ptc-amino acid analyzer. The Hitachi analyzer will be used by many NIH-funded research investigators at UTMB; a representative user group, with 14 funded NIH grants, is described that includes five major and five minor NIH-funded users, who include multidisciplinary investigators from several departments at UTMB, e.g. Human Biological Chemistry and Genetics, Microbiology and Toxicology, Preventive Medicine and Community Health, and the Marine Biomedical Institute. Examples of biomedical questions or diseases under investigation by these users include gastrointestinal bacterial pathogenesis, xenobiotics, diabetic and senile cataractogenesis, environmental toxicology and mutagenesis. The amino acid analyzer will be operated and maintained in the Protein Chemistry Core within the UTMB Biomolecular Resource Facility, a longstanding research support facility that includes five cores, namely, Protein Chemistry, Peptide Synthesis, Protein Expression and Purification, Mass Spectrometry, and Proteomics. Together, these Cores provide a wide range of research services for the entire University campus, including multiple campus research centers. Acquisition of the requested instrumentation will significantly enhance the ability of NIH-funded investigators to conduct their ongoing hypothesis-driven research and, moreover, will extend their research opportunities in the future to higher levels of productivity and scientific impact.

描述（由申请人提供）： 请求支持日立 L-8800 型氨基酸分析仪，以便为加尔维斯顿德克萨斯大学医学分部 (UTMB) 相对较大的生物医学研究人员群体进行广泛的氨基酸分析。日立分析仪采用柱后方法，利用比色检测（茚三酮）或荧光检测（邻苯二甲醛）。此外，L-8800 可以执行多种分析方法，包括蛋白质/肽水解产物、生理液体和特殊样品的水解产物，例如纤维蛋白或经过化学或翻译后修饰的蛋白质。蛋白质/肽浓度的成分分析和精确分析以及生理液体和组织提取物样品中游离氨基酸的分析将是日立分析仪极其重要的应用之一。所要求的日立分析仪将取代已退役 15 年的 Beckman 6300 和已老化 13 年的 Applied Biosystems 420H Ptc 氨基酸分析仪。 Hitachi 分析仪将被 UTMB 的许多 NIH 资助的研究人员使用；描述了一个具有 14 项 NIH 资助的代表性用户组，其中包括 5 个由 NIH 资助的主要用户和 5 个次要用户，其中包括来自 UTMB 多个部门的多学科研究人员，例如人类生物化学和遗传学、微生物学和毒理学、预防医学和社区健康以及海洋生物医学研究所。这些用户正在研究的生物医学问题或疾病的例子包括胃肠道细菌发病机制、异生素、糖尿病和老年性白内障发生、环境毒理学和诱变。氨基酸分析仪将在 UTMB 生物分子资源设施内的蛋白质化学核心中运行和维护，这是一个长期的研究支持设施，包括五个核心，即蛋白质化学、肽合成、蛋白质表达和纯化、质谱和蛋白质组学。这些核心共同为整个大学校园（包括多个校园研究中心）提供广泛的研究服务。获得所需的仪器将显着增强 NIH 资助的研究人员进行正在进行的假设驱动研究的能力，此外，还将扩大他们未来的研究机会，以提高生产力和科学影响力。