Symphony Multiplex Peptide Synthesizer w/ VISION Workstn

带 VISION Workstn 的 Symphony 多重肽合成器

• 批准号: 6733418
• 负责人: KENNETH G MANN
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6733418
• 关键词: biomedical equipment purchase; combinatorial chemistry; high performance liquid chromatography; peptide chemical synthesis; protein purification

DESCRIPTION (provided by applicant): This request is for an automated peptide synthesizer with a combinatorial reactor and a dedicated preparative HPLC. We have chosen the Symphony Multiplex from Protein Technologies Inc., as the peptide synthesizer and the VISION Workstation from Applied Biosystems as the preparative HPLC system. The Biochemistry Department at the University of Vermont has maintained a peptide synthesis capacity since 1984. The most recent instrument, a Chemtech model 90, has operated since 1993 is at the end of its lifetime and has limited capacity to support multiple investigators. There are multiple researchers at the University of Vermont with the need for peptides and with this award we intend to establish a core facility that would make peptides primarily for core users. We have requested the Symphony Multiplex because it has the capabilities to meet the needs of the research activities of multiple NIH funded research programs which include the development of reagents for antibody development, the evaluation of super-molecular organization of protein-ligand complexes, cellular regulation studies including protein-nucleic acid interactions, blood coagulation mechanism, muscle function, signal transduction, antigen-antibody interactions, amino acid libraries, and novel synthetic enzymes. The peptide synthesis system will be incorporated into the Department of Biochemistry's analytical facility in the College of Medicine. Financial support will be provided by the Department of Biochemistry, the College of Medicine, the Vermont Cancer Center, and by user fees. The principle investigator, Dr. Kenneth Mann and co-principle investigator Dr. Robert Hondal have used peptide synthesis extensively for antibody development, studies of protein interaction and protein semi-synthesis. This instrument will be under the primary control of Dr. Hondal who will be assisted by a technical staff with expertise in the use of the protein synthesizer and other analytical tools in the facility. In addition, this instrument facility will be incorporated into the training enterprise for graduate students in Biochemistry, Chemistry and other departments at the University of Vermont. The preparative HPLC system will be used by the facility to purify peptides and other biomolecules. There is currently no preparative HPLC system on the campus of the University of Vermont and this system is badly needed for many investigators.

描述（由申请人提供）：此请求是针对带有组合反应器和专用制备型 HPLC 的自动肽合成仪。我们选择 Protein Technologies Inc. 的 Symphony Multiplex 作为肽合成仪，选择 Applied Biosystems 的 VISION Workstation 作为制备型 HPLC 系统。佛蒙特大学生物化学系自 1984 年以来一直保持着肽合成能力。最新的仪器 Chemtech 90 型自 1993 年开始运行，现已达到其使用寿命，支持多名研究人员的能力有限。佛蒙特大学有多名研究人员需要肽，通过该奖项，我们打算建立一个核心设施，主要为核心用户生产肽。我们请求 Symphony Multiplex 是因为它能够满足多个 NIH 资助的研究项目的研究活动需求，其中包括抗体开发试剂的开发、蛋白质-配体复合物超分子组织的评估、细胞调控研究包括蛋白质-核酸相互作用、凝血机制、肌肉功能、信号转导、抗原-抗体相互作用、氨基酸库和新型合成酶。该肽合成系统将并入医学院生物化学系的分析设施。财务支持将由生物化学系、医学院、佛蒙特州癌症中心和使用费提供。首席研究员 Kenneth Mann 博士和联合首席研究员 Robert Hondal 博士已将肽合成广泛用于抗体开发、蛋白质相互作用和蛋白质半合成的研究。该仪器将由 Hondal 博士主要控制，他将得到具有使用蛋白质合成器和设施中其他分析工具专业知识的技术人员的协助。此外，该仪器设施将并入佛蒙特大学生物化学、化学等系研究生培训企业。该设施将使用制备型 HPLC 系统来纯化肽和其他生物分子。目前佛蒙特大学校园内没有制备型 HPLC 系统，但许多研究人员急需该系统。