Instrumentation for Peptide Synthesis Facility

肽合成设施仪器

• 批准号: 6577546
• 负责人: KATHRYN IVANETICH
• 金额: $ 24.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6577546
• 关键词: biomedical equipment purchase; high performance liquid chromatography; nucleic acid purification; nucleic acid sequence; protein purification; protein sequence

DESCRIPTION (provided by applicant): The Health Sciences campus of the University of California at San Francisco is one of the foremost centers of biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have an exceptional record in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators' needs in DNA and peptide sequencing, synthesis, analysis and purification. It is housed in 1,900 sq. ft. of laboratory/office space. Approximately 225 principal investigators have used the facility in the last year. Approximately 190 PIs have had 31,150 DNA templates sequenced, 105 PIs had ca. 3,100 oligonucleotides synthesized, over 30 PIs have had 155 peptides synthesized, and approximately 20 PIs had approximately 50 proteins sequenced. The facility is overseen by a Board of Overseers. Dr. Kathryn Ivanetich is the Director and oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 433A peptide synthesizer, Rainin Symphony Multiplex multiple peptide synthesizer, a Shimadzu analytical and preparative HPLC systems. The instruments are requested to replace outdated and obsolete instrumentation, and to provide increased automation to provide peptide synthesis, analysis and purification quickly, inexpensively and reliably to UCSF investigators. This request is supported by eleven UCSF Principal Investigators with considerable, ongoing PHS/NIH grant support. These PIs represent 8 UCSF departments, including Biochemistry and Biophysics, Medicine, Anatomy, Microbiology and Immunology, Physiology, Anesthesia, and Laboratory Medicine, plus the Cardiovascular Research Institute. The requested instrumentation would be a truly shared resource. No single investigator, group of investigators or department would dominate its use.

描述（由申请人提供）：加州大学旧金山分校健康科学校区是世界上最重要的生物医学研究中心之一。其医学院、药学院、牙科学院和护理学院聘用了数百名杰出的研究人员，他们在获得 NIH 奖项方面有着杰出的记录。加州大学旧金山分校校园拥有最先进的生物技术核心设施——生物分子资源中心 (BRC)。 BRC 自 1985 年起为 UCSF 校园提供服务，满足研究人员在 DNA 和肽测序、合成、分析和纯化方面的需求。它坐落在 1,900 平方英尺的实验室/办公空间内。去年约有 225 名主要研究人员使用过该设施。大约 190 个 PI 对 31,150 个 DNA 模板进行了测序，105 个 PI 对大约 31,150 个 DNA 模板进行了测序。合成了 3,100 个寡核苷酸，超过 30 个 PI 合成了 155 个肽，大约 20 个 PI 对大约 50 个蛋白质进行了测序。该设施由监督委员会监督。 Kathryn Ivanetich 博士担任主任，负责监督 BRC 的日常科学、财务和行政事务。申请资助Applied Biosystems 433A 肽合成仪、Rainin Symphony Multiplex 多肽合成仪、Shimadzu 分析和制备型 HPLC 系统。这些仪器被要求取代过时和过时的仪器，并提供更高的自动化程度，以便为 UCSF 研究人员快速、廉价且可靠地提供肽合成、分析和纯化。这一请求得到了 11 名 UCSF 首席研究员的支持，并获得了大量持续的 PHS/NIH 拨款支持。这些 PI 代表 UCSF 的 8 个院系，包括生物化学和生物物理学、医学、解剖学、微生物学和免疫学、生理学、麻醉学和检验医学，以及心血管研究所。所请求的仪器将是真正的共享资源。任何一个调查员、一组调查员或一个部门都不会主导其使用。