DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDYING BIOCHEMICAL REACTIONS

研究生化反应的生物物理方法的发展

• 批准号: 3843292
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3843292
• 关键词: acid base balance; bioengineering /biomedical engineering; biomedical equipment development; blood /plasma substitute; carbon; carbon dioxide; carbon monoxide; chemical binding; chemical reaction; chlorine; hemoglobin; human tissue; infrared spectrometry; microcalorimetry; nuclear magnetic resonance spectroscopy; phosphates; stable isotope; temperature

Work on the development of instrumentation for the evaluation of various hemoglobin derivatives proposed as a blood substitute has proceeded at an accelerated rate with the coming on line of the pilot plant at LAIR which can furnish large amounts of the protein. The oxygen equilibrium curve apparatus is now being tested on human hemoglobin samples and calorimetric measurements of the binding of CO,CO2, and chloride to HbAo and the DBBF- HB product are underway. The pulsed accelerated flow apparatus is nearly ready for operation. Of considerable importance in evaluating the product's suitability for injection into humans is the purity of the material, a precise knowledge of the oxygen equilibrium curve and the protein's response to its various effectors, i.e. chloride, pH, temperature, phosphate, CO2, as well as oxygen concentration. Binding isotherms of oxygen and carbon monoxide in the presence and absence of these effectors are being determined both manometrically and optically as well as in the microcalorimeter. CO2 binding curves are being determined calorimetrically and by C13 NMR. The physical chemistry of hemoglobin has suffered in the past from the lack of a suitable standard state from which to start the free energy calculations. These studies on the isoionic protein, i.e. in the absence of all effectors, will allow such a standard state to be established.

为评估各种仪器开发的工作 提议作为血液替代的血红蛋白衍生物已在 加速率，lair的飞行员工厂的即将到来 可以提供大量蛋白质。 氧平衡曲线 现在正在对人类血红蛋白样品和量热量的设备进行测试 CO，CO2和氯化物与HBAO和DBBF-的结合的测量 HB产品正在进行中。 脉冲加速流动设备几乎是 准备运行。 在评估 产品对人类注入的适合性是纯度 材料，氧平衡曲线的精确知识和 蛋白质对其各种效应子的反应，即氯化物，pH， 温度，磷酸盐，二氧化碳以及氧气浓度。 结合 在存在和不存在的情况下，氧和一氧化碳的等温线 这些效应子在手持和光学上都被确定为 以及在微升力仪中。 二氧化碳结合曲线被确定 量热法和C13 NMR。 血红蛋白的物理化学具有 过去由于缺乏合适的标准状态而受苦 开始自由能计算。 这些关于同志的研究 蛋白质，即在没有所有效应子的情况下，将允许这样的标准 要建立的状态。