DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDYING BIOCHEMICAL REACTIONS

研究生化反应的生物物理方法的发展

• 批准号: 3843292
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3843292
• 关键词: acid base balance; bioengineering /biomedical engineering; biomedical equipment development; blood /plasma substitute; carbon; carbon dioxide; carbon monoxide; chemical binding; chemical reaction; chlorine; hemoglobin; human tissue; infrared spectrometry; microcalorimetry; nuclear magnetic resonance spectroscopy; phosphates; stable isotope; temperature

Work on the development of instrumentation for the evaluation of various hemoglobin derivatives proposed as a blood substitute has proceeded at an accelerated rate with the coming on line of the pilot plant at LAIR which can furnish large amounts of the protein. The oxygen equilibrium curve apparatus is now being tested on human hemoglobin samples and calorimetric measurements of the binding of CO,CO2, and chloride to HbAo and the DBBF- HB product are underway. The pulsed accelerated flow apparatus is nearly ready for operation. Of considerable importance in evaluating the product's suitability for injection into humans is the purity of the material, a precise knowledge of the oxygen equilibrium curve and the protein's response to its various effectors, i.e. chloride, pH, temperature, phosphate, CO2, as well as oxygen concentration. Binding isotherms of oxygen and carbon monoxide in the presence and absence of these effectors are being determined both manometrically and optically as well as in the microcalorimeter. CO2 binding curves are being determined calorimetrically and by C13 NMR. The physical chemistry of hemoglobin has suffered in the past from the lack of a suitable standard state from which to start the free energy calculations. These studies on the isoionic protein, i.e. in the absence of all effectors, will allow such a standard state to be established.

致力于开发各种评估仪器 提议作为血液替代品的血红蛋白衍生物已在 随着 LAIR 试点工厂的上线，速度加快了 可以提供大量的蛋白质。 氧平衡曲线 目前正在对人体血红蛋白样本和热量测定装置进行测试 测量 CO、CO2 和氯化物与 HbAo 的结合以及 DBBF- HB产品正在进行中。 脉冲加速流装置几乎 准备运行。 对于评价具有相当重要的意义 产品是否适合注射到人体取决于其纯度 材料、氧平衡曲线的精确知识和 蛋白质对其各种效应物的反应，即氯、pH、 温度、磷酸盐、CO2 以及氧气浓度。 装订 在存在和不存在的情况下氧气和一氧化碳的等温线 这些效应器通过测压和光学方法确定为 以及微热量计。 CO2 结合曲线正在确定 量热法和 C13 NMR。 血红蛋白的物理化学性质 过去由于缺乏合适的标准状态而遭受痛苦 开始自由能计算。 这些关于等离子的研究 蛋白质，即在没有所有效应器的情况下，将允许这样的标准 状态待建立。