DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MACROMOLECULAR REACTIONS

研究生物大分子反应的生物物理方法的发展

• 批准号: 3942821
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942821
• 关键词: acidity /alkalinity; adenosinetriphosphatase; cell membrane; chemical reaction; enzyme substrate; fiber optics; hemoglobin; lasers; muscle contraction; sickle cell anemia; stop flow technique; thermodynamics; viscosity

The adaptation of the isoionic hemoglobin preparation developed in this Laboratory by the joint Forces Casualty Care Blood Resources Division based at Letterman Army Research Institute has provided stimulus to a renewed interest in several instruments developed in this laboratory. A modified oxygen binding to hemoglobin apparatus has been set up and is presently undergoing tests in the Laboratory of Molecular Biology, NIA. A highly modified cell is nearing completion of which will permit the measurement of the oxygen dissociation curve of hemoglobin simultaneously by a manometric method and spectroscopy. Such data are needed if the various models of how hemoglobin binds oxygen are to be critically tested. In collaboration with computational scientists at UCLA and DCRT a considerable effort has been mounted to have all of the needed programs operating on the local IBM AT rather than a main frame computer. The inertial drive quench flow apparatus and thermal-optical stopped flow apparatus have had a number of improvements made to them over the last year and it is expected that the quench flow machine will go operational in the laboratory of Biological Chemistry, NIA by the end of the summer. It has become clear that for the thermal stopped flow to be useful a differential version will need to be built and this should be finished in the next year. Experiments are planned on the ATPase ATP reactions of muscle as a test of the system.

开发等离子血红蛋白制剂的适应性 在联合部队伤亡护理血液实验室 莱特曼陆军研究所资源部 刺激了人们对多种工具的新兴趣 是在这个实验室开发的。 修饰的氧结合 血红蛋白仪已经建立，目前正在进行 测试在 NIA 分子生物学实验室进行。 一个高度 改造后的电池即将完成，这将允许 血红蛋白氧解离曲线的测量 同时通过测压法和光谱法。 这样的 如果血红蛋白如何结合的各种模型需要数据 氧气必须经过严格测试。 与合作 加州大学洛杉矶分校 (UCLA) 和 DCRT 的计算科学家 已付出努力来完成所有需要的计划 在本地IBM AT而不是主机上运行 电脑。 惯性驱动骤冷流动装置和热光 停流装置进行了许多改进 去年对他们来说，预计淬火流 机器将在生物实验室投入使用 化学，NIA 夏末。 已经明朗了 对于热停止流动有用的差异 需要构建版本，这应该在下一个版本中完成 年。 计划对 ATPase ATP 反应进行实验 肌肉作为系统的测试。