DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MACROMOLECULAR REACTIONS

研究生物大分子反应的生物物理方法的发展

• 批准号: 3942821
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942821
• 关键词: acidity /alkalinity; adenosinetriphosphatase; cell membrane; chemical reaction; enzyme substrate; fiber optics; hemoglobin; lasers; muscle contraction; sickle cell anemia; stop flow technique; thermodynamics; viscosity

The adaptation of the isoionic hemoglobin preparation developed in this Laboratory by the joint Forces Casualty Care Blood Resources Division based at Letterman Army Research Institute has provided stimulus to a renewed interest in several instruments developed in this laboratory. A modified oxygen binding to hemoglobin apparatus has been set up and is presently undergoing tests in the Laboratory of Molecular Biology, NIA. A highly modified cell is nearing completion of which will permit the measurement of the oxygen dissociation curve of hemoglobin simultaneously by a manometric method and spectroscopy. Such data are needed if the various models of how hemoglobin binds oxygen are to be critically tested. In collaboration with computational scientists at UCLA and DCRT a considerable effort has been mounted to have all of the needed programs operating on the local IBM AT rather than a main frame computer. The inertial drive quench flow apparatus and thermal-optical stopped flow apparatus have had a number of improvements made to them over the last year and it is expected that the quench flow machine will go operational in the laboratory of Biological Chemistry, NIA by the end of the summer. It has become clear that for the thermal stopped flow to be useful a differential version will need to be built and this should be finished in the next year. Experiments are planned on the ATPase ATP reactions of muscle as a test of the system.

均质血红蛋白制剂的适应 在这个实验室里，联合部队伤亡护理血液 莱特曼陆军研究所的资源部门 已经为几种乐器带来了重新兴趣的刺激 在这个实验室中开发。 修饰的氧结合到 血红蛋白设备已建立，目前正在进行 NIA分子生物学实验室的测试。 高度 修改的单元将接近完成，这将允许 血红蛋白的氧离解曲线的测量 同时通过平衡方法和光谱法。 这样的 如果血红蛋白如何结合的各种模型，则需要数据 氧气应进行严格测试。 与 UCLA和DCRT的计算科学家 已经努力拥有所有必要的程序 在本地IBM上运行，而不是主框架 电脑。 惯性驱动淬灭流仪和热光学 停止流动设备进行了许多改进 去年对他们来说，预计会淬火 机器将在生物学实验室中运营 化学，NIA到夏季结束。 这已经很清楚了 对于热停止流程的有用的差分 版本将需要构建，该版本应在下一个版本中完成 年。 计划在ATPase ATP反应上进行实验 肌肉作为系统的测试。