DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES

开发用于溶液和细胞生化研究的生物量热计

• 批准号: 3878934
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3878934
• 关键词: NAD nucleosidase; microcalorimetry; phospholipids; reagent /indicator; thermodynamics

Studies of the reaction of NAD-NADASE have been extended on the all tantalum stopped-flow microcalorimeter to 10 degrees C, 25 degrees C, and 37 degrees C confirming the endothermic nature of the reaction by demonstrating the 5-fold increase in activity from 25 degrees C to 10 degrees C with the almost complete loss in activity at 37 degrees C. A new heat capacity measuring microcalorimeter has been developed which is capable of measuring charges of 1 part in one hundred thousand of total heat capacity of phospholipids. A reaction heat of 60 calories/mole was found as they went from multilamellar vesicles to unilamellar vesicles. The total change in heat capacity was 2 to 3% depending on the system.

NAD-NADASE 反应的研究已扩展到所有 钽停流微量热计至 10°C、25°C 和 37°C 确认反应的吸热性质 证明从 25 摄氏度到 10 摄氏度，活性增加了 5 倍 摄氏度，在 37 摄氏度时活性几乎完全丧失。 研制出一种新型热容测量微热量计 能够测量总量十万分之一的电荷 磷脂的热容。反应热为 60 卡路里/摩尔 当它们从多层囊泡转变为单层囊泡时发现。这 热容量的总变化为 2% 至 3%，具体取决于系统。