DEVELOPMENT OF A PULSED LIGHT SOURCE, SINGLE PHOTON

脉冲光源、单光子的开发

• 批准号: 4694543
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4694543
• 关键词: analytical chemistry; biomedical equipment development; europium; immunology; lasers; photochemistry; radiotracer

The ability to detect minute amounts of protein is of interest both in protein chemistry and immunology. A possible replacement for radioimmuno assay (RIA) is the tagging of the protein by a caged molecule of Europium. By taking advantage of the very long fluorescence lifetime of Europium (500 microseconds) extreme sensitivities are possible. A pulsed nitrogen laser operating at 337 nm at 15 pps and power output of 200 microjoules been used with a 1/2 inch end of PET photomultiplier tube to examine a simple Europium tagged system.

检测微量蛋白质的能力引起了人们的兴趣 蛋白质化学和免疫学。 放射免疫的可能替代品 测定 (RIA) 是用铕笼状分子对蛋白质进行标记。 通过利用铕极长的荧光寿命（500 微秒）极端的灵敏度是可能的。 脉冲氮激光器 工作波长为 337 nm，速率为 15 pps，输出功率为 200 微焦耳 用 1/2 英寸端的 PET 光电倍增管来检查简单的 铕标记系统。