DEVELOPMENT OF RAMAN SPECTROSCOPY FOR ENZYME-SUBSTRATE REACTIONS

酶-底物反应拉曼光谱的发展

• 批准号: 3858004
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858004
• 关键词: Raman spectrometry; adenosine diphosphate; adenosine triphosphate; bioengineering /biomedical engineering; chemical reaction; enzyme mechanism; enzyme substrate analog; enzyme substrate complex; infrared spectrometry; magnesium; time resolved data

Raman Spectra of ATP and ADP were obtained at Sandia National Laboratories and at the University of New Mexico. These provide baseline spectra as we explore the feasibility of obtaining spectra of ATP and various ATP complexes and analogs using time resolved Raman Spectroscopy. We have shown that Raman difference spectra provide the sensitivity required for small perturbations in two relatively dilute samples such as the ATP complex with Mg and in comparison to ADP. The differences were known from infra-red spectroscopy which is done at very high phosphate concentration where complexing with other ATP or ADP molecules sometimes occurs making interpretation difficult.

ATP 和 ADP 的拉曼光谱在桑迪亚国家实验室获得 和新墨西哥大学。 这些提供了我们的基线光谱 探讨获取ATP及各种ATP谱图的可行性 使用时间分辨拉曼光谱法合成配合物和类似物。 我们有 表明拉曼差异光谱提供了所需的灵敏度 两个相对稀释的样品（例如 ATP）中的小扰动 与 Mg 形成络合物并与 ADP 进行比较。 这些差异是从 在非常高的磷酸盐浓度下完成的红外光谱 有时会与其他 ATP 或 ADP 分子发生络合 解释困难。