APPLICATIONS OF NEW MASS SPECTRAL TECHNIQUES

新质谱技术的应用

• 批准号: 3838039
• 负责人: J A KELLEY
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3838039
• 关键词: DNA; DNA damage; adduct; analytical chemistry; chemical structure; crosslink; drug interactions; ion exchange chromatography; liquid chromatography; mass spectrometry; method development; mitomycin C; nucleic acid structure; nucleosides; nucleotides; surfactant; trace elements

The objective of this project is research on and development of new mass spectral techniques in order to provide innovative and/or more rapid solutions to problems involving (1) chemical structure determination, (2) complex mixture analysis and (3) measurement of trace components in biological systems. Fast atom bombardment mass spectrometry and combined liquid chromatography-mass spectrometry remain the techniques of current interest. Both anion exchange and reverse phase microcolumn liquid chromatography have been combined with a continuous-flow fast atom bombardment ionization interface for the analysis of nucleosides, nucleotides and DNA adducts. Use of quaternary amine surfactants during static and continuous-flow fast atom bombardment greatly enhances sensitivity for analysis of nucleotides. Combined liquid chromatography-mass spectrometry with electrospray ionization has been employed for the successful structural confirmation of a novel mitomycin C-DNA intrastrand cross-link.

该项目的目的是研究和发展新群众 光谱技术为了提供创新和/或更快的速度 解决涉及（1）化学结构确定的问题的解决方案，（2） 复杂的混合物分析和（3）测量痕量成分 生物系统。 快速原子轰炸质谱和合并 液相色谱 - 质谱谱图仍然是电流的技术 兴趣。 阴离子交换和反相微柱液体 色谱已与连续流的快速原子结合 用于分析核苷的轰击电离界面， 核苷酸和DNA加合物。 使用第四纪胺表面活性剂 静态而连续流的快速原子轰击会大大增强 核苷酸分析的灵敏度。 合并液体 带有电喷雾电离的色谱 - 质量光谱法已经 用于成功确认新型丝裂霉素的结构确认 C-DNA内链交联。