APPLICATIONS OF NEW MASS SPECTRAL TECHNIQUES

新质谱技术的应用

• 批准号: 3774555
• 负责人: J A KELLEY
• 金额: --
• 依托单位: DIVISION OF CANCER TREATMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774555
• 关键词: analytical chemistry; chemical structure; enzyme inhibitors; ion exchange chromatography; liquid chromatography; mass spectrometry; method development; nucleic acid structure; nucleosides; nucleotides; surfactant; trace elements

The objective of this project is research on and development of new mass spectral techniques in order to provide innovative and/or more rapid solutions to problems involving (1) chemical structure determination, (2) complex mixture analysis and (3) measurement of trace components in biological systems. Fast atom bombardment mass spectrometry and combined liquid chromatography-mass spectrometry remain the techniques of current interest. Reversed phase, paired-ion techniques are being developed for combining micro-column liquid chromatography with continuous-flow fast atom bombardment ionization for the on-line liquid chromatography-mass spectrometry analysis of nucleosides. Use of acidic, anionic surfactants during static fast atom bombardment greatly enhances sensitivity for analysis of cytosine nucleosides such as cyclopentenyl cytosine. The structure of an in vitro metabolite of benzene-3-carboxamide riboside, which is a potent inhibitor of inosine monophosphate dehydrogenase, has been confirmed as the adenine dinucleotide conjugate by negative ion fast atom bombardment mass spectrometry.

该项目的目的是研究和发展新群众 光谱技术为了提供创新和/或更快的速度 解决涉及（1）化学结构确定的问题的解决方案，（2） 复杂的混合物分析和（3）测量痕量成分 生物系统。 快速原子轰炸质谱和合并 液相色谱 - 质谱谱图仍然是电流的技术 兴趣。 正在开发相反的阶段，配对的离子技术 将微牙液色谱与连续流相结合 在线液相色谱质量的原子轰击电离 核苷的光谱分析。 使用酸性，阴离子表面活性剂 在静态快速原子轰击中，大大提高了对 分析胞嘧啶核苷，例如环戊烯基胞嘧啶。 这 苯-3-羧化酰胺核苷的体外代谢产物的结构， 这是一种有效的肌苷一磷酸脱氢酶的抑制剂，具有 被确认为腺苷二核苷酸偶联物，负离子快速 原子轰炸质谱。