PHYLOGENETICS OF MOCCASINS (PIT VIPERS)--IMPROVING ANTIVENIN SPECIFICITY

鹿皮鞋 (PIT VIPERS) 的系统发育学--提高抗蛇毒素特异性

• 批准号: 3915860
• 负责人: DAVID J MORAFKA
• 金额: --
• 依托单位: CALIFORNIA STATE UNIV-DOMINGUEZ HILLS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3915860
• 关键词: alleles; antivenins; biological polymorphism; evolution; field study; gene expression; isozymes; reptile poison; snakes; tissue /cell culture

This proposal is a continuation of our investigation of rattlesnake (genera Sistrurus and Crotalus) genealogy utilizing data from morphology, SEM scale surface analysis, G and C banded karyotypes, DNA hybridization, and most intensely, gel electrophoresis of allozymes. Drawing upon a previously resolved evolutionary tree, we will analyze possible hybridization between sister species of rattlesnakes (using the above techniques) and reassess their taxonomic status. Our efforts will then address those pit vipers most closely related to the rattlesnakes, the moccasins (genera Aokistrodon, Hypernale, Dienagkistrodon, and Calloselasma). The same techniques will be employed to establish the phylogenetic position of these genera and their species, first relative to other pit vipers, and then relatives to each other, thereby generating the first cladistic evolutionary tree ever generated for these organisms. The resulting tree should provide a valuable resource for toxicologists searching for natural groups of species - in order to develop sets of monoclonal antibodies, or tot draw up as pharmacological resources for medically valuable enzymes. All student trainees will be rotated through each of the five major protocols. Library Search training will precede and computer analysis will follow these rotations. Each student will be assigned particular short term investigations leading to prompt publication. As a result they should develop competence (and self-confidence) in a wide rage of biomedically valuable skills. A cost efficient group presentation of prior student work is planned for the First World Herpetology Congress in London, and a second trip to China, hosted by our sister University in Wuhan, will give MBRS students access to 55% of all our required snake species on a single excursion.

该建议是我们对响尾蛇的调查的延续 （sistrus and crotalus属）使用形态学的数据，谱系， SEM量表表面分析，G和C带核型，DNA杂交， 最强烈的是同酶的凝胶电泳。吸引一个 以前解决的进化树，我们将分析可能 响尾蛇姐妹物种之间的杂交（使用上述 技术）并重新评估其分类状态。然后我们的努力将 解决与响尾蛇最紧密相关的那些毒蛇 Moccasins（Aokistrodon属，Hypernale，Dienagkistrodon和 calloselasma）。将采用相同的技术来建立 这些属及其物种的系统发育位置，第一相对 到其他维普斯，然后彼此亲戚，从而产生 这些生物有史以来生成的第一个cladistic进化树。 最终的树应为毒理学家提供宝贵的资源 寻找自然物种群 - 为了开发一组 单克隆抗体或TOT作为药理学资源 医学上有价值的酶。 所有学生学员都将通过五个专业的每一个旋转 协议。图书馆搜索培训将在之前和计算机分析之前 将遵循这些旋转。每个学生将被分配 短期调查导致迅速发布。结果他们 应该在广泛的愤怒中发展能力（和自信心） 生物善良有价值的技能。经济高效的小组演示 计划在第一世界的疱疹学大会上工作 伦敦，第二次前往中国，由我们的姊妹大学主持 武汉将使MBRS学生获得我们所有必需蛇的55％ 一次游览中的物种。