The Procurement of BioAnalyzers to Expedite and Enhance Services Provided by the National Natural Toxins Research Center

采购生物分析仪以加快和增强国家天然毒素研究中心提供的服务

• 批准号: 10808337
• 负责人: Elda E. Sanchez
• 金额: $ 16.4万
• 依托单位: TEXAS A&M UNIVERSITY-KINGSVILLE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10808337
• 关键词: Animals; Antibodies; Antivenins; Applied Research; Area; Biochemical; Biochemistry; Biocompatible Materials; Biological; Biological Assay; Biology; Biomedical Research; Blood Coagulation Factor; Characteristics; Coagulation Process; Collection; Complex; Development; Discipline; Drug Design; Ecology; Ecosystem; Equipment; Foundations; Growth; Hematology; Immunoassay; International; Methods; Microscope; Molecular Probes; Mus; Natural Products; Pharmaceutical Preparations; Pharmacology; Physiological; Process; Reagent; Reproducibility; Research; Research Personnel; Resources; Role; Sampling; Services; Snake Bites; Snake Venoms; Snakes; System; Therapeutic; Therapeutic Uses; Time; Tissues; Toxin; Translational Research; United States National Institutes of Health; Venoms; antibody conjugate; biological research; biomarker identification; cold temperature; improved; instrument; instrumentation; multidisciplinary; novel therapeutics; outreach program; parent grant; programs; protein biomarkers; research study; service programs; synthetic polymer Bioplex

PROJECT SUMMARY: Natural products have been a foundation of drug design, and snakes and other animal venoms have vast potential. Snake venoms have provided molecular probes that have been used to decipher numerous complex physiological and pathophysiological processes and have served as the starting point for developing several important classes of drugs. The venom and anti-venom research fields depend on access to reliable and reproducible venom-related resources developed by the National Natural Toxin Research Center (NNTRC). The NNTRC requires equipment and instrumentation for storing crude snake venoms, venom fractions, purified toxins, cloned products, snake and mice tissues, critical reagents (i.e., biochemical assays and reagents, antibodies, conjugated antibodies, and biological samples) used for diverse translational research. The equipment and instrumentation being requested, two ultra-low temperature freezers, a Bio-Plex 200 Multiplex Array System, a DxH 500 Hematology Analyzer, a Stago STA Compact Coagulation Analyzer, and a DM1000 LED upright microscope, in the proposal are ideally suited to support critical research for maintaining the biological samples for long-term storage, analyzing biochemical protein markers in a multiplex format that provide more information from less sample volume in less time than traditional immunoassay methods, determining the hematological variables and coagulation parameters for the characteristic hematological abnormalities observed in snake envenomation. The specific aims of the parent grant are to: 1. Operate the National Natural Toxins Research Center as a resource center that provides high- quality venom and products that support biological and biomedical research to national and international programs. 2. Develop and expand the collection of snakes, specialized services, and outreach programs to support the growth of venom-related research in the U.S. 3. Conduct a state-of-the-art applied research program to support the development of new venom- related research services. Access to this equipment and instruments will enable the NNTRC to fulfill its specific aims and allow researchers to make significant advances in the search for venom-derived therapeutics. RELEVANCE: Venom research is a diverse and multidisciplinary field that encompasses a range of scientific areas, including biochemistry, pharmacology, ecology, and conservation biology. It could lead to the discovery of new drugs, better antivenoms, and an improved understanding of venomous animals and their role in ecosystems. The resources of the NNTRC have been applied to research in a wide range of these disciplines, research that has been supported by multiple NIH I/C’s, the NSF, the Dept of Defense, and national and international research agencies.

项目概要： 天然产物一直是药物设计的基础，蛇和其他动物的毒液具有广泛的应用前景。 蛇毒提供了分子探针，已被用来破译许多信息。 复杂的生理和病理生理过程，并作为 开发几类重要的药物取决于毒液和抗毒液研究领域。 获得国家天然毒素开发的可靠且可重复的毒液相关资源 研究中心 (NNTRC)。 NNTRC 需要用于储存粗蛇的设备和仪器。 毒液、毒液成分、纯化毒素、克隆产品、蛇和小鼠组织、关键试剂（即 生化测定和试剂、抗体、缀合抗体和生物样品）用于 多样化的转化研究所要求的设备和仪器，两项超低。 冷冻柜、Bio-Plex 200 多重阵列系统、DxH 500 血液分析仪、Stago 提案中的 STA 紧凑型凝血分析仪和 DM1000 LED 正置显微镜是理想的选择 适合支持维持生物样本长期储存、分析的关键研究 多重格式的生化蛋白质标记物可通过更少的样品量提供更多信息 比传统免疫测定方法更短的时间，确定血液学变量和 蛇中观察到的特征性血液学异常的凝血参数 家长资助的具体目标是： 1. 将国家天然毒素研究中心作为资源中心运营，提供高水平的 支持国家和生物医学研究的优质毒液和产品 国际项目。 2. 开发和扩大蛇的收集、专业服务和外展计划，以 支持美国毒液相关研究的发展 3. 开展最先进的应用研究计划，支持新毒液的开发 相关研究服务。 使用这些设备和仪器将使 NNTRC 能够实现其特定目标并允许 研究人员在寻找毒液衍生疗法方面取得重大进展。 关联： 毒液研究是一个多元化的多学科领域，涵盖一系列科学领域， 包括生物化学、药理学、生态学和保护生物学。 新药、更好的抗生素以及对有毒动物及其作用的进一步了解 NNTRC 的资源已广泛应用于这些领域的研究。 多个 NIH I/C、NSF、国防部和 国家和国际研究机构。

项目成果

Rattling the border wall: Pathophysiological implications of functional and proteomic venom variation between Mexican and US subspecies of the desert rattlesnake Crotalus scutulatus.

• DOI: 10.1016/j.cbpc.2017.10.008
• 发表时间: 2018-03
• 期刊: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
• 作者: Dobson J;Yang DC;Op den Brouw B;Cochran C;Huynh T;Kurrupu S;Sánchez EE;Massey DJ;Baumann K;Jackson TNW;Nouwens A;Josh P;Neri-Castro E;Alagón A;Hodgson WC;Fry BG
• 通讯作者: Fry BG

The characterization of trans-pecos copperhead (Agkistrodon contortrix pictigaster) venom and isolation of two new dimeric disintegrins.

跨佩科斯铜头蛇 (Agkistrodon contortrix pictigaster) 毒液的表征和两种新二聚解整合素的分离。

• DOI: 10.1016/j.biologicals.2016.02.005
• 发表时间: 2016
• 期刊: Biologicals : journal of the International Association of Biological Standardization
• 作者: Lucena,Sara;Rodríguez-Acosta,Alexis;Grilli,Elyse;Alfonso,Andrea;Goins,Amber;Ogbata,Ifunanya;Walls,Robert;Suntravat,Montamas;Uzcátegui,NestorL;Guerrero,Belsy;Sánchez,EldaE
• 通讯作者: Sánchez,EldaE

Coral snake antivenom produced in chickens (Gallus domesticus).

• DOI: 10.1590/s0036-46652014000100009
• 发表时间: 2014-01
• 期刊: Revista do Instituto de Medicina Tropical de Sao Paulo
• 影响因子: 1.9
• 作者: Aguilar I;Sánchez EE;Girón ME;Estrella A;Guerrero B;Rodriguez-Acosta FA
• 通讯作者: Rodriguez-Acosta FA

Characterization of the Rabbit as an In Vitro and In Vivo Model to Assess the Effects of Fibrinogenolytic Activity of Snake Venom on Coagulation.

• DOI: 10.1111/bcpt.12848
• 发表时间: 2018-01
• 期刊: Basic & clinical pharmacology & toxicology
• 影响因子: 3.1
• 作者: Nielsen VG;Sánchez EE;Redford DT
• 通讯作者: Redford DT

Biochemical and biological characterisation of lancehead (Bothrops venezuelensis Sandner 1952) snake venom from the Venezuelan Central Coastal range.

委内瑞拉中部海岸范围的矛头蛇 (Bothrops venezuelensis Sandner 1952) 蛇毒的生化和生物学特征。

• 发表时间: 2014
• 期刊: Boletin de malariologia y salud ambiental
• 作者: Sánchez,EldaE;Girón,MaríaE;Uzcátegui,NestorL;Guerrero,Belsy;Saucedo,Max;Cuevas,Esteban;Rodríguez-Acosta,Alexis
• 通讯作者: Rodríguez-Acosta,Alexis