The Procurement of BioAnalyzers to Expedite and Enhance Services Provided by the National Natural Toxins Research Center

采购生物分析仪以加快和增强国家天然毒素研究中心提供的服务

• 批准号: 10808337
• 负责人: Elda E. Sanchez
• 金额: $ 16.4万
• 依托单位: TEXAS A&M UNIVERSITY-KINGSVILLE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10808337
• 关键词: Animals; Antibodies; Antivenins; Applied Research; Area; Biochemical; Biochemistry; Biocompatible Materials; Biological; Biological Assay; Biology; Biomedical Research; Blood Coagulation Factor; Characteristics; Coagulation Process; Collection; Complex; Development; Discipline; Drug Design; Ecology; Ecosystem; Equipment; Foundations; Growth; Hematology; Immunoassay; International; Methods; Microscope; Molecular Probes; Mus; Natural Products; Pharmaceutical Preparations; Pharmacology; Physiological; Process; Reagent; Reproducibility; Research; Research Personnel; Resources; Role; Sampling; Services; Snake Bites; Snake Venoms; Snakes; System; Therapeutic; Therapeutic Uses; Time; Tissues; Toxin; Translational Research; United States National Institutes of Health; Venoms; antibody conjugate; biological research; biomarker identification; cold temperature; improved; instrument; instrumentation; multidisciplinary; novel therapeutics; outreach program; parent grant; programs; protein biomarkers; research study; service programs; synthetic polymer Bioplex

PROJECT SUMMARY: Natural products have been a foundation of drug design, and snakes and other animal venoms have vast potential. Snake venoms have provided molecular probes that have been used to decipher numerous complex physiological and pathophysiological processes and have served as the starting point for developing several important classes of drugs. The venom and anti-venom research fields depend on access to reliable and reproducible venom-related resources developed by the National Natural Toxin Research Center (NNTRC). The NNTRC requires equipment and instrumentation for storing crude snake venoms, venom fractions, purified toxins, cloned products, snake and mice tissues, critical reagents (i.e., biochemical assays and reagents, antibodies, conjugated antibodies, and biological samples) used for diverse translational research. The equipment and instrumentation being requested, two ultra-low temperature freezers, a Bio-Plex 200 Multiplex Array System, a DxH 500 Hematology Analyzer, a Stago STA Compact Coagulation Analyzer, and a DM1000 LED upright microscope, in the proposal are ideally suited to support critical research for maintaining the biological samples for long-term storage, analyzing biochemical protein markers in a multiplex format that provide more information from less sample volume in less time than traditional immunoassay methods, determining the hematological variables and coagulation parameters for the characteristic hematological abnormalities observed in snake envenomation. The specific aims of the parent grant are to: 1. Operate the National Natural Toxins Research Center as a resource center that provides high- quality venom and products that support biological and biomedical research to national and international programs. 2. Develop and expand the collection of snakes, specialized services, and outreach programs to support the growth of venom-related research in the U.S. 3. Conduct a state-of-the-art applied research program to support the development of new venom- related research services. Access to this equipment and instruments will enable the NNTRC to fulfill its specific aims and allow researchers to make significant advances in the search for venom-derived therapeutics. RELEVANCE: Venom research is a diverse and multidisciplinary field that encompasses a range of scientific areas, including biochemistry, pharmacology, ecology, and conservation biology. It could lead to the discovery of new drugs, better antivenoms, and an improved understanding of venomous animals and their role in ecosystems. The resources of the NNTRC have been applied to research in a wide range of these disciplines, research that has been supported by multiple NIH I/C’s, the NSF, the Dept of Defense, and national and international research agencies.

项目摘要： 天然产品一直是药物设计的基础，蛇和其他动物毒液含有疫苗 潜在的。蛇毒液提供了用于破译无数的分子问题 复杂的生理和病理生理过程，已成为 开发几种重要类别的药物。毒液和抗毒液研究领域取决于 获取由国家天然毒素开发的可靠和再现与毒液相关的资源 研究中心（NNTRC）。 NNTRC需要设备和仪器来存储粗蛇 毒液，毒液分数，纯化的毒素，克隆产物，蛇和小鼠组织，临界试剂（即， 用于生化测定和试剂，抗体，偶联抗体和生物样品） 多样化的翻译研究。请求设备和仪器，两个超低 温度冷冻器，Bio-plex 200多重阵列系统，DXH 500血液学分析仪，stago 在提案中，STA紧凑型凝血分析仪和DM1000 LED直立显微镜是理想情况下 适合支持关键研究以维持长期存储的生物样品，并进行了分析 多重格式的生化蛋白质标记物提供了更多信息来自样本体积的更多信息 比传统的免疫测定方法更少的时间，确定血液学变量和 在蛇中观察到的特征血液学异常的凝血参数 毒化。父母赠款的具体目的是： 1。运营国家天然毒素研究中心作为提供高级资源中心 优质毒液和支持国家和生物医学研究的产品 国际计划。 2。开发和扩展蛇，专业服务和外展计划的收集 支持美国与毒液有关的研究的增长 3.进行最先进的应用研究计划，以支持开发新的毒液 相关研究服务。 使用该设备和工具将使NNTRC能够实现其特定目标并允许 研究人员在寻找毒液衍生的疗法方面取得了重大进展。 关联： 毒液研究是一个潜水和多学科领域，包括一系列科学领域， 包括生物化学，药理学，生态学和保护生物学。它可能导致发现 新药，更好的抗抗蛇毒以及对有毒动物的改善的理解及其在 生态系统。 NNTRC的资源已应用于其中的广泛研究 学科，由多个NIH I/C，NSF，国防部和 国家和国际研究机构。

项目成果

Rattling the border wall: Pathophysiological implications of functional and proteomic venom variation between Mexican and US subspecies of the desert rattlesnake Crotalus scutulatus.

• DOI: 10.1016/j.cbpc.2017.10.008
• 发表时间: 2018-03
• 期刊: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
• 作者: Dobson J;Yang DC;Op den Brouw B;Cochran C;Huynh T;Kurrupu S;Sánchez EE;Massey DJ;Baumann K;Jackson TNW;Nouwens A;Josh P;Neri-Castro E;Alagón A;Hodgson WC;Fry BG
• 通讯作者: Fry BG

The characterization of trans-pecos copperhead (Agkistrodon contortrix pictigaster) venom and isolation of two new dimeric disintegrins.

跨佩科斯铜头蛇 (Agkistrodon contortrix pictigaster) 毒液的表征和两种新二聚解整合素的分离。

• DOI: 10.1016/j.biologicals.2016.02.005
• 发表时间: 2016
• 期刊: Biologicals : journal of the International Association of Biological Standardization
• 作者: Lucena,Sara;Rodríguez-Acosta,Alexis;Grilli,Elyse;Alfonso,Andrea;Goins,Amber;Ogbata,Ifunanya;Walls,Robert;Suntravat,Montamas;Uzcátegui,NestorL;Guerrero,Belsy;Sánchez,EldaE
• 通讯作者: Sánchez,EldaE

Coral snake antivenom produced in chickens (Gallus domesticus).

• DOI: 10.1590/s0036-46652014000100009
• 发表时间: 2014-01
• 期刊: Revista do Instituto de Medicina Tropical de Sao Paulo
• 影响因子: 1.9
• 作者: Aguilar I;Sánchez EE;Girón ME;Estrella A;Guerrero B;Rodriguez-Acosta FA
• 通讯作者: Rodriguez-Acosta FA

Characterization of the Rabbit as an In Vitro and In Vivo Model to Assess the Effects of Fibrinogenolytic Activity of Snake Venom on Coagulation.

• DOI: 10.1111/bcpt.12848
• 发表时间: 2018-01
• 期刊: Basic & clinical pharmacology & toxicology
• 影响因子: 3.1
• 作者: Nielsen VG;Sánchez EE;Redford DT
• 通讯作者: Redford DT

Biochemical and biological characterisation of lancehead (Bothrops venezuelensis Sandner 1952) snake venom from the Venezuelan Central Coastal range.

委内瑞拉中部海岸范围的矛头蛇 (Bothrops venezuelensis Sandner 1952) 蛇毒的生化和生物学特征。

• 发表时间: 2014
• 期刊: Boletin de malariologia y salud ambiental
• 作者: Sánchez,EldaE;Girón,MaríaE;Uzcátegui,NestorL;Guerrero,Belsy;Saucedo,Max;Cuevas,Esteban;Rodríguez-Acosta,Alexis
• 通讯作者: Rodríguez-Acosta,Alexis