BEHAVIORAL ROLES OF PEPTIDES: PHYSIOLOGY AND BIOPHYSICS

肽的行为作用：生理学和生物物理学

基本信息

批准号：
3476764
负责人：
MARK D KIRK
金额：
$ 7.91万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-08-01 至 1993-03-31
项目状态：
已结题

项目摘要

The long term goal of the proposed research is to understand how the central nervous system mediates animal behavior, and to elucidate the functional roles of neuropeptides in the control of behavior. The complexity of the vertebrate nervous system generally precludes a detailed cellular analysis of even the simplest behaviors. Therefore, my approach is to utilize the marine mollusc Aplysia californica with its relatively simple nervous system and stereotyped behaviors as a model to study nervous function. I propose to use a unique, multilevel approach in my laboratory to pursue the functions of neuropeptides in the modulation of feeding behavior, a behavior of general interest and one for which several aspects of its organization are already known. The three levels of investigation are: 1) Multiple intracellular and extracellular recordings in semi- intact and reduced preparations will be used to study peptidergic modulation of the neuronal circuits that mediate feeding behavior. These experiments will be combined with in vivo recordings of feeding muscles during different modes of feeding and during different states of feeding arousal to correlate the various forms of neuronal modulation with overt changes in the animal's feeding behavior. 2) Feeding motoneurons and interneurons will be identified and tested for the synthesis of peptide neurotransmitters using a double labeling technique that combines intracellular lucifer yellow dye-filling and immunohistochemistry to test for peptide immunoreactivity. Once peptidergic neurons are identified, their influence on target neurons and/or muscles will be investigated to determine the contribution of released peptides to their postsynaptic effects. 3) Finally, the changes in ionic currents and channels, and second messengers which are responsible for peptidergic modulation in the feeding system will be studied using the voltage clamp and patch clamp techniques. This research will provide an understanding of peptidergic modulation from the level of biophysical actions to the cellular mediation of behavior.

拟议研究的长期目标是了解中枢神经系统介导动物行为，并阐明神经肽在控制中的功能作用行为。脊椎动物神经系统的复杂性通常排除了即使最简单的行为。因此，我的方法是利用海洋软体动物Aplysia加州及其相对简单神经系统和定型行为作为研究的模型神经功能。我建议使用独特的多级方法在我的实验室中追求神经肽在调节喂养行为，一种普遍兴趣的行为和该组织的几个方面已经存在已知。调查的三个级别是： 1）半细胞内和细胞外记录完整和减少的准备工作将用于研究介导的神经元电路的肽吉尼调节喂养行为。这些实验将与在不同模式下喂养肌肉的体内记录喂食和在不同状态的喂养状态将各种形式的神经元调制与动物喂养行为的明显变化。 2）将确定喂养运动神经元和神经元，并测试了使用A的肽神经递质合成的结合细胞内路西法的双重标记技术黄色染料填充和免疫组织化学测试肽免疫反应性。一旦肽能神经元确定，它们对目标神经元和/或肌肉的影响将进行调查以确定释放出其突触后作用的肽。 3）最后，离子电流和通道的变化以及第二个负责肽能的使者喂养系统中的调制将使用电压夹和斑块夹技术。这项研究将提供对肽能的理解从生物物理作用水平到细胞的调节行为的调解。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Localization of catecholamines in the buccal ganglia of Aplysia californica.

儿茶酚胺在加州海兔颊神经节中的定位。

DOI：
10.1016/0006-8993(88)90512-4
发表时间：
1988
期刊：
Brain research
影响因子：
2.9
作者：
Rathouz,MM;Kirk,MD
通讯作者：
Kirk,MD

Neuromuscular organization of the buccal system in Aplysia californica.

加州海兔颊系统的神经肌肉组织。

DOI：
10.1002/cne.903120204
发表时间：
1991
期刊：
The Journal of comparative neurology
影响因子：
0
作者：
Scott,ML;Govind,CK;Kirk,MD
通讯作者：
Kirk,MD

Peptidergic motoneurons in the buccal ganglia of Aplysia californica: immunocytochemical, morphological, and physiological characterizations.

海兔颊神经节的肽能运动神经元：免疫细胞化学、形态学和生理学特征。

DOI：
10.1007/bf00198352
发表时间：
1991
期刊：
Journal of comparative physiology. A, Sensory, neural, and behavioral physiology
影响因子：
0
作者：
Church,PJ;Cohen,KP;Scott,ML;Kirk,MD
通讯作者：
Kirk,MD

Premotor neurons in the feeding system of Aplysia californica.

海兔进食系统中的运动前神经元。