FUNCTIONAL NEURAL REGENERATION IN APLYSIA

海兔的功能性神经再生

• 批准号: 3417761
• 负责人: MARK D KIRK
• 金额: $ 10.51万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-03 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3417761
• 关键词: Aplysia; axon; eating; electron microscopy; electrophysiology; ethology; immunocytochemistry; nervous system regeneration; neuronal guidance; nutrition related tag; oral behavior; synapses; videotape /videodisc

We propose to study functional regeneration in the feeding system of the gastropod mollusc, Aplysia californica. The first specific aim is to ascertain the capacity for recovery of consummatory feeding behavior following bilateral crushes to central nerve tracts that connect the cerebral and buccal ganglia. Behavioral parameters will be measured from direct observations and from videotaped images of subjects taken following the lesion to quantify the recovery of biting, swallowing, and rejection. The second specific aim is to determine if regeneration of connections, by identified neurons involved in consummatory feeding, accompanies behavioral recovery, and to test if these connections are normal. For this specific aim we will use simultaneous multiple intracellular and extracellular recordings and stimulation. the third specific aim is to test for changes in cellular properties in response to CNS injury and during functional regeneration using immunocytochemistry, electrophysiological recordings, and electron microscopy. The proposed studies will provide answers to fundamental questions about neuronal repair and functional regeneration in vivo. It is likely that some cellular mechanisms of neuronal repair evolved in ancestors common to molluscs and mammals; if so, our results will be applicable to higher vertebrate organisms, including humans, where regeneration in the CNS is more limited. An important aspect of this proposal is the combination of cellular and behavioral studies. With this integrated approach, we can begin to describe the contribution by sensory, pattern initiator (i.e., command), and modulatory neural systems to behavioral recovery in terms of functional regeneration of specific neuronal connections.

我们建议研究饲喂系统的功能再生 腹足类软体动物，海兔。 第一个具体目标是 确定完全喂养行为的恢复能力 双侧挤压连接大脑的中枢神经束后 大脑和颊神经节。 行为参数将通过测量 直接观察和以下拍摄对象的录像图像 损伤部位以量化咬合、吞咽和排斥反应的恢复情况。 第二个具体目标是确定连接是否再生，通过 确定了参与完成喂养的神经元，伴随着行为 恢复，并测试这些连接是否正常。 对于这个具体的 目标我们将同时使用多个细胞内和细胞外 录音和刺激。 第三个具体目标是测试变化 响应中枢神经系统损伤和功能期间的细胞特性 使用免疫细胞化学、电生理记录进行再生， 和电子显微镜。 拟议的研究将为以下基本问题提供答案： 体内神经元修复和功能再生。 很可能是这样 神经元修复的一些细胞机制是在祖先中共同进化而来的 软体动物和哺乳动物；如果是这样，我们的结果将适用于更高的 脊椎动物，包括人类，中枢神经系统的再生是 更有限。 该提案的一个重要方面是将 细胞和行为研究。 通过这种综合方法，我们可以 开始描述感官、模式引发者的贡献（即 命令），以及调节神经系统对行为恢复的影响 特定神经元连接的功能再生。