FUNCTIONAL NEURAL REGENERATION IN APLYSIA

海兔的功能性神经再生

• 批准号: 2268806
• 负责人: MARK D KIRK
• 金额: $ 11.93万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-03 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2268806
• 关键词: Aplysia; alternatives to animals in research; axon; eating; electron microscopy; electrophysiology; ethology; immunocytochemistry; nervous system regeneration; neuronal guidance; nutrition related tag; oral behavior; synapses; videotape /videodisc

We propose to study functional regeneration in the feeding system of the gastropod mollusc, Aplysia californica. The first specific aim is to ascertain the capacity for recovery of consummatory feeding behavior following bilateral crushes to central nerve tracts that connect the cerebral and buccal ganglia. Behavioral parameters will be measured from direct observations and from videotaped images of subjects taken following the lesion to quantify the recovery of biting, swallowing, and rejection. The second specific aim is to determine if regeneration of connections, by identified neurons involved in consummatory feeding, accompanies behavioral recovery, and to test if these connections are normal. For this specific aim we will use simultaneous multiple intracellular and extracellular recordings and stimulation. the third specific aim is to test for changes in cellular properties in response to CNS injury and during functional regeneration using immunocytochemistry, electrophysiological recordings, and electron microscopy. The proposed studies will provide answers to fundamental questions about neuronal repair and functional regeneration in vivo. It is likely that some cellular mechanisms of neuronal repair evolved in ancestors common to molluscs and mammals; if so, our results will be applicable to higher vertebrate organisms, including humans, where regeneration in the CNS is more limited. An important aspect of this proposal is the combination of cellular and behavioral studies. With this integrated approach, we can begin to describe the contribution by sensory, pattern initiator (i.e., command), and modulatory neural systems to behavioral recovery in terms of functional regeneration of specific neuronal connections.

我们建议研究在喂养系统中的功能再生 加利福尼亚州Aplysia的腹足动物软体动物。 第一个具体目的是 确定恢复完整喂养行为的能力 遵循双边粉碎到连接的中央神经区 大脑和颊神经节。 行为参数将根据 直接观察和从以下主题拍摄的录像图像 量化咬伤，吞咽和排斥的病变。 第二个具体目的是确定连接的再生是否 鉴定出参与完整喂养的神经元，伴随行为 恢复，并测试这些连接是否正常。 对于这个特定 目的我们将同时使用多个细胞内和细胞外 记录和刺激。 第三个具体目的是测试更改 在响应中枢神经系统损伤和功能期间的细胞性质中 使用免疫细胞化学，电生理记录的再生， 和电子显微镜。 拟议的研究将为有关的基本问题提供答案 神经元修复和体内功能再生。 很可能 神经元修复的某些细胞机制在共有的祖先中演变为 软体动物和哺乳动物；如果是这样，我们的结果将适用于更高 脊椎动物的生物，包括人类，中枢神经系统的再生是 更有限。 该提案的一个重要方面是结合 细胞和行为研究。 采用这种综合方法，我们可以 开始描述感官，模式引发剂的贡献（即 命令）和调节神经系统以行为恢复 特定神经元连接的功能再生。