COLONIZATION MECHANISMS OF PERIODONTAL PATHOGENS

牙周病原体的定植机制

• 批准号: 3462320
• 负责人: Richard J Lamont
• 金额: $ 9.26万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3462320
• 关键词: Bacteroides gingivalis; SDS polyacrylamide gel electrophoresis; Streptococcus sanguis; adhesin; affinity chromatography; bacterial cytopathogenic effect; chemical binding; dental plaque; enzyme linked immunosorbent assay; high performance liquid chromatography; immune complex; ion exchange chromatography; laboratory mouse; laboratory rabbit; microorganism culture; microorganism growth; microorganism interaction; molecular cloning; monoclonal antibody; pellicle; periodontitis; protein purification; protein sequence; proteolysis; receptor binding; recombinant DNA; saliva; tooth surface; western blottings

Dental plaque is a precursor of periodontal disease, one of the most common bacterial infections of man. The factors that regulate the colonization of plaque by periodontal pathogens are poorly understood. Adherence of the pathogenic bacteria to early plaque bacteria is an important first step in the colonization process. In this proposal, the adherence of Bacteroides gingivalis (a putative periodontal pathogen) to Streptococcus sanguis (an early plaque species) will be investigated. The bacteroides will be radiolabelled and binding to S. sanguis immobilized on a solid support measured. The nature of the binding will be ascertained by testing for inhibition with various physical and chemical agents. Interacting molecules will be visualized by incubating labelled organisms of one bacterial species with surface molecules extracted from its binding partner and separated by electrophoresis and blotting. The bacterial molecules involved in binding will be isolated by various chromatographic and electrophoretic techniques and/or recombinant DNA methodologies. After chemical characterization of the interacting molecules, they will be used for the production of monocional antibodies. Antibodies that inhibit binding of the homologous bacteria will be used to define the functional domains of the interacting molecules. Thus, these studies will begin to dissect the interbacterial interactions important in the colonization of the oral cavity by pathogenic organisms. This information will enhance our understanding of how periodontal pathogens establish themselves in the mouth. The elucidation of these mechanisms may allow the development of measures to prevent the colonization of these bacteria thus inhibiting the development of periodontal disease.

牙菌斑是牙周病的先兆，是最常见的牙周病之一。 人类常见的细菌感染。 调节因素 人们对牙周病原体在菌斑上的定植知之甚少。 致病菌对早期牙菌斑细菌的粘附是 殖民进程中重要的第一步。 在该提案中， 牙龈拟杆菌（一种假定的牙周病原体）的粘附 将研究血链球菌（一种早期菌斑物种）。 拟杆菌将被放射性标记并与血链球菌结合 固定在测量的固体支持物上。 约束力的性质 通过测试各种物理和抑制的抑制来确定 化学制剂。 通过孵育可以使相互作用的分子可视化 用表面分子标记一种细菌物种的生物体 从其结合伴侣中提取并通过电泳分离 印迹。 参与结合的细菌分子将被分离 通过各种色谱和电泳技术和/或 重组DNA方法学。 经过化学表征后 相互作用的分子，它们将用于生产 单克隆抗体。 抑制结合的抗体 同源细菌将用于定义其功能域 相互作用的分子。 因此，这些研究将开始剖析 细菌间相互作用对于口腔定植很重要 病原生物造成的空腔。 这些信息将增强我们的 了解牙周病原体如何在牙周组织中生存 嘴。 阐明这些机制可能有助于开发 采取措施防止这些细菌定植，从而抑制 牙周病的发展。