ANTITUMOR, ANTIVIRAL AGENTS IN MICROALGAE

微藻中的抗肿瘤、抗病毒剂

基本信息

批准号：
3194365
负责人：
YUZURU SHIMIZU
金额：
$ 19.21万
依托单位：
UNIVERSITY OF RHODE ISLAND
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-06-01 至 1994-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3194365
关键词：
Mastigophora X ray crystallography algae antiinfective agents antineoplastics antiviral agents cytotoxicity mass spectrometry medicinal plants microorganism culture nuclear magnetic resonance spectroscopy

项目摘要

This proposal is to investigate microalgae, especially dinoflagellates, for novel antitumor, antiviral and antimicrobial agents. The research is also intended to determine if these microalgae are the primary sources of highly active interesting molecules recently discovered in marine invertebrates, and thus can be reliable sources of these potentially important therapeutic agents. Anticipated compounds include polyethers, macrolides, and other polyoxygenated compounds. The proposal is based on the recent findings of the occurrence of these compounds in dinoflagellates. Specifically, both free-living and symbiotic or associated organisms will be isolated and cultured in large scale. Emphasis will be on freshly isolated strains from natural habitats or hosts, although those held in culture collections and already in our possession will be also investigated. The cells and broths will be extracted, and the extracts will be fractionated guided by bioassays. The structures of purified compounds will be studied by physico-chemical methods including high field NMR, mass spectroscopy, and X-ray crystallography. Antitumor and antiviral assays will be conducted by Bristol-Myers Co., and NCI, and in- house cytotoxicity tests will be performed with limited cell lines as routine isolation guides. Antimicrobial tests will be done in house.

该提案旨在研究微藻，特别是甲藻，用于新型抗肿瘤、抗病毒和抗菌药物。该研究是还旨在确定这些微藻是否是最近在海洋中发现的高度活跃的有趣分子无脊椎动物，因此可以成为这些潜在的可靠来源重要的治疗剂。预期的化合物包括聚醚、大环内酯类和其他多氧化化合物。该提案基于最近发现这些化合物出现在甲藻。具体来说，自由生活的生物体和共生或相关的生物体都会进行大规模分离培养。重点将放在新鲜上从自然栖息地或宿主中分离出的菌株，尽管那些保存在我们已经拥有的文化收藏品也将调查了。将提取细胞和肉汤，并且提取物将在生物测定的指导下进行分级。纯化后的结构化合物将通过物理化学方法进行研究，包括高场核磁共振、质谱和 X 射线晶体学。抗肿瘤和抗病毒检测将由百时美施贵宝公司和 NCI 进行，并在室内细胞毒性测试将使用有限的细胞系进行常规隔离指南。抗菌测试将在内部进行。