Biochemical characterisation of the translocation process of RxLR-like effector proteins via tyrosine-O-sulphate modified cell surface receptors

RxLR样效应蛋白通过酪氨酸-O-硫酸盐修饰的细胞表面受体易位过程的生化表征

• 批准号: BB/J018333/1
• 负责人: Pieter Van West
• 金额: $ 46.01万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ018333%2F1
• 关键词: Biochemical; characterisation; translocation; process; RxLR; Biochemical; characterisation; translocation; process; RxLR

The United Nations predict that by 2050 about 9-10 billion people will populate the world. To provide enough food for the growing world population requires that areas of food production that still have the capacity to grow, like aquaculture, will be exploited more rigorously in the future but also that losses due to diseases need to be reduced. Our aim is to understand early host pathogen interactions of oomycete parasites that heavily impact food production. Oomycetes are fungal-like organisms that cause diseases on plants and animals. For example different Saprolegnia species infects fish (Saprolegniosis) at different development levels. In order to cause disease these parasites need to deliver molecules into their host cells to manipulate the host in such a way that it can infect and propagate successfully. One group of such molecules are the so called effectors. These molecules do not only target specific host molecules but some also seem to highjack host transport mechanisms in order to enter the cells. We will characterise the molecular mechanisms that effector proteins use to translocate into host cells, since a detailed understanding of these processes will help us to develop methods/substances that can block the infection process. This will allow fish to fight off their oomycete attackers more efficiently. In addition, we would like to use the 'effector entering system' to develop an immersion vaccine whereby fish do not need to be handled and injected anymore. Such a vaccine would reduce stress in fish and the occurrence of vaccination associated diseases, which includes Saprolegniosis.

联合国预测，到2050年，大约9-100亿人将占世界。为了为不断增长的世界人口提供足够的食物，要求仍然具有种植能力的粮食生产领域，例如水产养殖，将来将更加严格地利用，但还需要减少由于疾病而造成的损失。我们的目的是了解卵巢寄生虫的早期宿主病原体相互作用，从而严重影响粮食生产。 Oomycetes是真菌样生物，会引起植物和动物的疾病。例如，不同的腐生物种会在不同的发育水平上感染鱼类（腐生）。为了引起疾病，这些寄生虫需要将分子传递到其宿主细胞中，以操纵宿主，以使其能够成功感染和传播。一组这样的分子是所谓的效应子。这些分子不仅靶向特定的宿主分子，而且有些分子似乎也是寄托宿主运输机制，以便进入细胞。我们将表征效应蛋白用来转移到宿主细胞中的分子机制，因为对这些过程的详细了解将有助于我们开发可以阻止感染过程的方法/物质。这将使鱼类能够更有效地抵抗卵形攻击者。此外，我们想使用“效应子进入系统”来开发一种浸入疫苗，从而不需要再处理和注射鱼类。这种疫苗会减轻鱼类的压力和疫苗接种相关疾病的发生，其中包括腐生性疾病。