Development of an immersion vaccine for salmonids

鲑鱼浸泡疫苗的开发

• 批准号: NE/P010873/1
• 负责人: Pieter Van West
• 金额: $ 25.78万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FP010873%2F1
• 关键词: Development; immersion; vaccine; salmonids; Development; immersion; vaccine; salmonids

Fish handling and injection-based vaccination is one of the biggest causes of stress for fish and always results in increased mortality due to post-vaccination outbreaks of saprolegniosis and bacterial infections. In Scotland alone, millions of pre-smolt (10% of all hatched salmon) die due to Saprolegnia infections after receiving an injection-based vaccine. Therefore, there is an urgent need to develop better vaccination practices to increase animal welfare and reduce mortalities. Immersion vaccination of fish was the first method used to demonstrate effective protection against bacterial diseases, but was overtaken by injection vaccination due to the need for inclusion of vaccine adjuvants. Should enhanced delivery of antigens be possible by the immersion route clearly this holds enormous promise to completely obviate fish handling, thus reducing stress and significantly lowering mortality. The central hypothesis of our programme is that immersion vaccination with recombinant SpHtp1-coupled antigens can be used successfully to protect fish against diseases. Our overall objective is to develop and commercialise the SpHtp1 translocation mechanism into an immersion vaccine delivery method for salmonids and other farmed fish. We have already demonstrated that our patented method can be used successfully and effectively in directing proteins into live fish. Furthermore, we have observed specific antibody production in trout that were immersed in a solution of SpHtp1 fused to mRFP.Therefore, our main outputs will be: 1) Evidence that the translocating peptide of SpHtp1 coupled to selected antigens give protection against disease of concern in salmonid aquaculture, in immersion vaccination trial experiments.2) Optimisation of the immune response in fish.3) A highly efficient and productive SpHtp1-recombinant protein overexpression method for large-scale production and future commercialisation of the vaccine method by our commercial partners BAHL and Pulcea Ltd.4) A method to recycle and inactivate the immersion vaccine that can be employed on site in the farm.All outputs will be exploited by BAHL and Pulcea Ltd. in obtaining licenses and approvals for commercialisation and eventually full use by the aquaculture industry. Antigens of essential pathogens are already available through BAHL, and further antigens will be sought in future projects by the UoA and BAHL. The current application is ambitious but achievable and will take 24 months to complete because of the significant amount of work that is proposed. In summary, the potential impact of an immersion vaccine for finfish is "aquaculture industry-transformational" as fish vaccinated by injection could be eliminated, which would greatly increase the welfare of the fish and minimise fish losses and thus financial losses in the aquaculture business worldwide.

鱼类处理和基于注射的疫苗接种是造成鱼类压力的最大原因之一，始终导致疾病后疫苗接种和细菌感染引起的死亡率增加。仅在苏格兰，由于收到基于注射的疫苗后，由于腐生性感染而导致的数百万张前烟（占所有孵化鲑鱼的10％）死亡。因此，迫切需要开发更好的疫苗接种实践，以增加动物福利并减少死亡率。浸入疫苗接种是一种用于证明有效保护细菌疾病的方法，但由于需要纳入疫苗辅助因素而被注射疫苗接种所取代。浸入式途径可能会增加抗原的递送，这清楚地表明，这具有巨大的承诺，可以完全消除鱼类的处理，从而减轻压力并显着降低死亡率。我们计划的中心假设是，可以成功使用重组SPHTP1偶联抗原的浸入式疫苗接种，以保护鱼类免受疾病的侵害。我们的总体目标是将SPHTP1易位机制开发和商业化为鲑鱼和其他养殖鱼的浸入疫苗输送方法。我们已经证明，我们的专利方法可以成功地有效地将蛋白质引导到活鱼中。此外，我们已经观察到鳟鱼中的特定抗体产生，这些抗体产生沉浸在融合到MRFP的SPHTP1的溶液中。因此，我们的主要输出将是：1）证据表明，SPHTP1偶联的SPHTP1肽与所选抗原的易位替代抗原的易位抗原对盐分含量的疾病的保护，对鲑鱼的疾病进行了危害3的疾病。我们的商业合作伙伴Bahl和Pulcea Ltd.4）一种可以在农场中实现商业化的运用，可以通过Bahl and Pulciative in verraive Incove and Pulcea and Pullciation。水产养殖业充分利用。基本病原体的抗原已经可以通过Bahl获得，UOA和BAHL将在未来的项目中寻求更多的抗原。当前的申请是雄心勃勃的，但可以实现，并且需要24个月的时间才能完成，因为提出了大量的工作。总而言之，浸入疫苗对芬鱼的潜在影响是“水产养殖行业变化”，因为可以消除注射疫苗的鱼类，这将大大增加鱼类的福利并最大程度地减少鱼类损失，从而在全球水产养殖业务中造成金融损失。