A systems biology based approach to functionally annotate and analyse the genome of the fish pathogenic oomycete Saprolegnia parasitica

基于系统生物学的方法，对鱼类致病性卵菌寄生水霉的基因组进行功能注释和分析

基本信息

批准号：
BB/G012075/1
负责人：
Pieter Van West
金额：
$ 40.86万
依托单位：
University of Aberdeen
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2009
资助国家：
英国
起止时间：
2009 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FG012075%2F1
关键词：
systems biology based approach functionally

项目摘要

Watermoulds, or oomycetes, contain some of the most devastating pathogens of animals and plants, causing enormous economic and environmental damage in natural and cultured ecosystems. The most destructive oomycete pathogens on fish are Saprolegnia parasitica and Saprolegnia diclina. These species are present in all fresh water habitats and represent a serious problem for the aquaculture industry, where it has been estimated that these oomycetes alone, kill over 10% of all hatched salmon and eggs. Watermoulds have several fungus-like characteristics but are not 'true fungi'. They belong to a group of organisms called the Stramenopiles or Heterokonts, which also include the golden-brown algae, kelp, and diatoms. Several clearly defined developmental stages are found in the life cycle of oomycetes that are not found in fungal pathogens, which makes them unique and it is becoming clear now that they have evolved different infection strategies compared to other parasites, fungal and bacterial pathogens. The asexual spore or sporangium is formed at the end of hyphal cells and can release many zoospores. These zoospores are able to swim as they have two flagella. Once the zoospore has produced a cell wall, it can germinate and produce infection hyphae. These hyphae can infect the skin and underlying tissue of the fish. Initially the head or the fins become infected and later the watermould is able to spread over the entire surface of the body. Disease is characterised by an external, cotton-like appearance with crescent-shaped or whorled patterns. Saprolegnia is able to cause cellular necrosis as well as dermal and epidermal damage. Infections do not appear to be tissue specific. Hyphae can also penetrate into the muscle and blood vessels of infected fish. In some cases infection takes place very rapidly and inflammatory responses in the fish appear to be absent. This has led several researchers to believe that S. parasitica is able to suppress the immune response in fish. In the current proposal, we will perform experiments that will help us to understand how this group of organisms cause disease. This in return will allow us to develop new sustainable ways of controling the disease. In order to analyse disease processes at the molecular level, it is necessary to first determine the coding regions of all genes of the pathogen. We can then use computers to identify genes likely to be involved in invading the fish and carry out experiments to see what the corresponding gene products (proteins) do. Furthermore we will use this information to see if the same genes in related organisms are used to attack both animals and plants. We will then investigate which genes are important for infection by studying when they are switched on during the development and infection cycles of the pathogen.

水龙头或卵菌中包含一些最具破坏性的动物和植物病原体，从而在自然和培养的生态系统中造成了巨大的经济和环境破坏。鱼类上最具破坏性的卵菌病原体是辣椒菌和saprolegnia diclina。这些物种都存在于所有淡水栖息地中，对于水产养殖业来说是一个严重的问题，据估计，仅这些卵菌，就杀死了所有孵化的鲑鱼和鸡蛋的10％以上。 Watermoulds具有几种真菌的特征，但不是“真正的真菌”。它们属于一组名为Stramenopiles或Heterokonts的生物，其中还包括金棕色藻类，海带和硅藻。在真菌病原体中找不到的几个明确定义的发育阶段在卵菌的生命周期中发现，这使得它们与众不同，而且现在它们已经与其他寄生虫，真菌和细菌病原体相比已经发展了不同的感染策略。无性孢子或孢子囊在菌丝细胞的末端形成，可以释放许多动态孢子。这些动物孢子可以游泳，因为它们有两个鞭毛。一旦动物孢子产生了一个细胞壁，它就会发芽并产生感染菌丝。这些菌丝可以感染鱼的皮肤和下层组织。最初，头部或鳍会被感染，后来水力可以散布在身体的整个表面上。疾病的特征是外观，带有新月形或磨牙图案的外观。腐生菌能够引起细胞坏死以及皮肤和表皮损伤。感染似乎不是组织特异性的。菌丝还可以渗透到感染鱼的肌肉和血管中。在某些情况下，感染发生非常快速，鱼类中的炎症反应似乎不存在。这使一些研究人员相信，寄生虫能够抑制鱼类的免疫反应。在当前的建议中，我们将进行实验，以帮助我们了解这组生物如何引起疾病。作为回报，这将使我们能够开发新的可持续方法来控制疾病。为了分析分子水平的疾病过程，有必要首先确定病原体所有基因的编码区域。然后，我们可以使用计算机来识别可能参与入侵鱼的基因并进行实验，以查看相应的基因产物（蛋白质）的作用。此外，我们将使用此信息来查看相关生物中相同的基因是否用于攻击动物和植物。然后，我们将研究哪些基因在病原体的发育和感染周期中何时开启，对感染很重要。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Distinctive expansion of potential virulence genes in the genome of the oomycete fish pathogen Saprolegnia parasitica.

DOI：
10.1371/journal.pgen.1003272
发表时间：
2013-06
期刊：
PLoS genetics
影响因子：
4.5
作者：
Jiang RH;de Bruijn I;Haas BJ;Belmonte R;Löbach L;Christie J;van den Ackerveken G;Bottin A;Bulone V;Díaz-Moreno SM;Dumas B;Fan L;Gaulin E;Govers F;Grenville-Briggs LJ;Horner NR;Levin JZ;Mammella M;Meijer HJ;Morris P;Nusbaum C;Oome S;Phillips AJ;van Rooyen D;Rzeszutek E;Saraiva M;Secombes CJ;Seidl MF;Snel B;Stassen JH;Sykes S;Tripathy S;van den Berg H;Vega-Arreguin JC;Wawra S;Young SK;Zeng Q;Dieguez-Uribeondo J;Russ C;Tyler BM;van West P
通讯作者：
van West P

A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss.

DOI：
10.1016/j.funbio.2014.04.008
发表时间：
2014-07
期刊：
FUNGAL BIOLOGY
影响因子：
2.5
作者：
Minor, Kirsty L.;Anderson, Victoria L.;Davis, Katie S.;Van Den Berg, Albert H.;Christie, James S.;Loebach, Lars;Faruk, Ali Reza;Wawra, Stephan;Secombes, Chris J.;Van West, Pieter
通讯作者：
Van West, Pieter

Parental transfer of the antimicrobial protein LBP/BPI protects Biomphalaria glabrata eggs against oomycete infections.

DOI：
10.1371/journal.ppat.1003792
发表时间：
2013
期刊：
PLoS pathogens
影响因子：
6.7
作者：
Baron OL;van West P;Industri B;Ponchet M;Dubreuil G;Gourbal B;Reichhart JM;Coustau C
通讯作者：
Coustau C

Emerging oomycete threats to plants and animals