STRUCTURES OF ANTIBIOTICS AND RELATED COMPOUNDS

抗生素及相关化合物的结构

• 批准号: 3124206
• 负责人: KENNETH L RINEHART
• 金额: $ 23.6万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3124206
• 关键词: Agrobacterium tumefaciens; Bacillus subtilis; Escherichia coli; Haemophilus influenzae; Herpes simplex disease; Porifera; RNA directed DNA polymerase; Saccharomyces cerevisiae; Tunicata; Vesiculovirus; algae; analytical chemistry; antibacterial agents; antibiotics; antifungal antibiotics; antineoplastic antibiotics; antiviral agents; chemical structure; drug screening /evaluation; hibernation; human immunodeficiency virus 1; immunomodulators; immunosuppressive; mass spectrometry; polyenes; stable isotope

Compounds with antiviral, antineoplastic, immunomodulatory, antifungal, and antibacterial activities will be identified in marine species and their structures will be assigned. These include antineoplastic compounds from Ecteinascidia turbinata and new components of the didemnin (antiviral, antineoplastic, immunosuppressive) complex from Trididemnum solidum, as well as compounds from other tunicates, sponges, and algae. Bioassays to be employed in identifying active extracts and isolated compounds include tests against Herpes simplex virus, type I, Vesicular stomatitis virus, visna virus, and HIV-1; reverse transcriptase; cytotoxicity assays with L1210 murine leukemia and CV-1 monkey kidney cells; brine shrimp assay and Agrobacterium tumefaciens potato tumor assay; B-cell and T-cell mitogenesis inhibition, mixed lymphocyte reaction; and antimicrobial assays against Bacillus subtilis, Micrococcus luteus, Escherichia coli, Saccharomyces cerevisiae, and Penicillium atrovenetum. Compounds with antiviral, immunomodulatory, and cytotoxic activities will also be sought and identified in extensive collections of cyanobacteria (blue-green algae), insects, and plant species. Structures of the antifungal polyene antibiotics hamycin and aureofungin will also be studied. In addition, structural studies will be carried out on pretunichlorins, unique nickel-containing chlorins from T. solidum, on hepatotoxins from cyanobacteria, on iron-transport hydroxamates from Hemophilus influenzae, and on a hibernation- and fasting-characteristic compound produced by black bears and humans.

具有抗病毒、抗肿瘤、免疫调节、抗真菌等作用的化合物 将鉴定海洋物种及其抗菌活性 将分配结构。 这些包括来自以下来源的抗肿瘤化合物： Ecteinascidia turbinata 和 didemnin 的新成分（抗病毒、 抗肿瘤、免疫抑制）复合物，来自 Triridemnum Solidum，如 以及来自其他被囊动物、海绵和藻类的化合物。 生物测定 用于鉴定活性提取物和分离的化合物包括 针对单纯疱疹病毒、I 型、水泡性口炎病毒、 维斯纳病毒和 HIV-1；逆转录酶；细胞毒性测定 L1210鼠白血病细胞和CV-1猴肾细胞；丰年虾测定和 根癌农杆菌马铃薯肿瘤测定； B 细胞和 T 细胞有丝分裂 抑制、混合淋巴细胞反应；和抗菌检测 枯草芽孢杆菌、藤黄微球菌、大肠杆菌、酵母菌 酿酒酵母和阿托维青青霉。 具有抗病毒、免疫调节和细胞毒活性的化合物将 也可以在广泛的蓝细菌收藏中寻找和鉴定 （蓝绿藻）、昆虫和植物物种。 的结构 抗真菌多烯抗生素 Hamycin 和 aureofungin 也将 研究过。 此外，还将对 pretunilorins 进行结构研究， 来自 T.solidum 的独特含镍二氢卟酚，对来自 T.solidum 的肝毒素 蓝细菌，对来自流感嗜血杆菌的铁转运异羟肟酸盐， 以及由黑色产生的具有冬眠和禁食特性的化合物 熊和人类。