STRUCTURES OF ANTIBIOTICS AND RELATED COMPOUNDS

抗生素及相关化合物的结构

• 批准号: 2750081
• 负责人: KENNETH L RINEHART
• 金额: $ 26.36万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-05-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2750081
• 关键词: Agrobacterium tumefaciens; Bacillus subtilis; Escherichia coli; Haemophilus influenzae; Herpes simplex disease; Micrococcus; Moniliales; Porifera; RNA directed DNA polymerase; Saccharomyces cerevisiae; Tunicata; Vesiculovirus; algae; alternatives to animals in research; analytical chemistry; antibacterial agents; antibiotics; antifungal antibiotics; antineoplastic antibiotics; antiviral agents; chemical structure; drug screening /evaluation; hibernation; human immunodeficiency virus 1; immunomodulators; immunosuppressive; mass spectrometry; polyenes; stable isotope

Compounds with antiviral, antineoplastic, immunomodulatory, antifungal, and antibacterial activities will be identified in marine species and their structures will be assigned. These include antineoplastic compounds from Ecteinascidia turbinata and new components of the didemnin (antiviral, antineoplastic, immunosuppressive) complex from Trididemnum solidum, as well as compounds from other tunicates, sponges, and algae. Bioassays to be employed in identifying active extracts and isolated compounds include tests against Herpes simplex virus, type I, Vesicular stomatitis virus, visna virus, and HIV-1; reverse transcriptase; cytotoxicity assays with L1210 murine leukemia and CV-1 monkey kidney cells; brine shrimp assay and Agrobacterium tumefaciens potato tumor assay; B-cell and T-cell mitogenesis inhibition, mixed lymphocyte reaction; and antimicrobial assays against Bacillus subtilis, Micrococcus luteus, Escherichia coli, Saccharomyces cerevisiae, and Penicillium atrovenetum. Compounds with antiviral, immunomodulatory, and cytotoxic activities will also be sought and identified in extensive collections of cyanobacteria (blue-green algae), insects, and plant species. Structures of the antifungal polyene antibiotics hamycin and aureofungin will also be studied. In addition, structural studies will be carried out on pretunichlorins, unique nickel-containing chlorins from T. solidum, on hepatotoxins from cyanobacteria, on iron-transport hydroxamates from Hemophilus influenzae, and on a hibernation- and fasting-characteristic compound produced by black bears and humans.

抗病毒，抗肿瘤，免疫调节，抗真菌和 抗菌活动将在海洋物种及其 结构将被分配。 这些包括来自 Ecteinascidia turbinata和Dodemnin的新成分（抗病毒，， 抗肿瘤，免疫抑制），来自Trididemn固体的复合物，如 以及来自其他膜，海绵和藻类的化合物。 生物测定到 用于识别活性提取物和孤立化合物中 针对单纯疱疹病毒的测试，I型，囊泡气孔病毒， Visna病毒和HIV-1；逆转录酶；细胞毒性测定 L1210鼠白血病和CV-1猴肾细胞；盐水虾测定法和 农杆菌土豆肿瘤分析； B细胞和T细胞有丝分裂发生 抑制作用，混合淋巴细胞反应；和抗菌测定 枯草芽孢杆菌，小叶片，大肠杆菌，糖疗法 酿酒酵母和青霉曲霉。 具有抗病毒，免疫调节和细胞毒性活性的化合物将 也可以在广泛的蓝细菌中寻求和鉴定 （蓝绿色藻类），昆虫和植物物种。 结构 抗真菌多烯抗生素Hamycin和Aureofungin也将是 研究。 此外，将在假设氯蛋白上进行结构研究， 来自T. solidum的独特含镍的氯蛋白，在肝毒素上 蓝细菌，对流感血友病的铁传输羟胺， 在冬眠和禁食特征上，黑色产生的化合物 熊和人类。