HIGH RESOLUTION MASS SPECTRA OF BIOORGANIC COMPOUNDS

生物有机化合物的高分辨率质谱

基本信息

批准号：
3274484
负责人：
KENNETH L RINEHART
金额：
$ 19.29万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
起止时间：
1979-05-01 至 1989-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3274484
关键词：
alkaloids analytical chemistry antibacterial agents antibiotics antineoplastic antibiotics antiviral agents biomedical automation biomedical equipment resource chemical structure clinical biomedical equipment clinical chemistry enzymes gangliosides gas chromatography glycolipids high performance liquid chromatography mass spectrometry neomycin nucleosides nucleotides organic chemicals pesticide residues pharmacology polyenes porphyrins pyrophosphates saltwater environment toxin metabolism

alkaloids analytical chemistry antibacterial agents antibiotics antineoplastic antibiotics antiviral agents biomedical automation biomedical equipment resource chemical structure clinical biomedical equipment clinical chemistry enzymes gangliosides gas chromatography glycolipids high performance liquid chromatography mass spectrometry neomycin nucleosides nucleotides organic chemicals pesticide residues pharmacology polyenes porphyrins pyrophosphates saltwater environment toxin metabolism

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项目摘要

High resolution mass spectrometry will be employed in studying biomedically important compounds, stressing the new technique of fast atom bombardment mass spectrometry (FABMS). A high field mass spectrometer will be modified to observe ions to m/z 10,000 at maximum accelerating potential (8KV). A variety of ion guns as well as postanalyzer acceleration will be employed to enhance sensitivity. Several matrices will be studied to enhance FAB ion production. Mass markers will be prepared to facilitate computerized data collection and accurate mass measurements of both positive and negative ions, and optimization of high resolution measurements will be studied in ion sources of different designs. Collisional activation will be employed to enhance FAB-induced fragmentation. A high performance liquid chromatograph will be coupled to a FAB mass spectrometer by a modified moving belt apparatus. Compounds to be studied by these techniques include involatile or thermally unstable antibiotics (especially peptides and polyenes), antiviral, antitumor and antimicrobial marine natural products, other peptides (enzymic digests of proteins, fluorogenic peptides, peptide derivatives of drugs and suicide inhibitors), polynucleotides, glycolipids, including gangliosides, coenzymes, terpene pyrophosphates, modified porphyrins, organometallic analogs of metalloenzymes, carcinogenic metabolites, organic compounds with hypervalent atoms, sulfonium salts, and metal carbonyls.

高分辨率质谱将用于生物医学研究重要化合物，强调快速原子轰击的新技术质谱法（FABM）。高场质谱仪将被修改在最大加速电势（8kV）下观察离子至m/z 10,000。一个将采用各种离子枪和后加速器加速提高灵敏度。将研究几个矩阵以增强FAB 离子生产。质量标记将做好准备以促进计算机化数据收集和准确的质量测量值负离子和高分辨率测量的优化将是在不同设计的离子源中进行了研究。碰撞激活将用于增强Fab诱导的碎片化。高性能液相色谱仪将与Fab质谱仪耦合改良的移动皮带设备。这些要研究的化合物技术包括投入或热不稳定的抗生素（尤其是肽和多烯），抗病毒，抗肿瘤和抗菌剂海洋天然产物，其他肽（蛋白质的酶消化，荧光蛋白肽，药物的肽衍生物和自杀抑制剂），）多核苷酸，糖脂，包括神经节苷脂，辅酶，萜烯焦磷酸盐，改良的卟啉，有机金属类似物的类似物金属酶，致癌代谢产物，有机化合物，具有高价值原子，硫盐和金属羰基。