HIGH RESOLUTION MASS SPECTRA OF BIOORGANIC COMPOUNDS

生物有机化合物的高分辨率质谱

• 批准号: 3274488
• 负责人: KENNETH L RINEHART
• 金额: $ 16.68万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3274488
• 关键词: Archaea; Haemophilus influenzae; antibiotics; antifungal antibiotics; antineoplastics; antiviral agents; biomedical equipment development; cardiotoxin; chlorin; cytochrome P450; enzyme structure; estrogen receptors; gamma aminobutyrate; glycolipids; glycosphingolipids; hemoprotein; hemoprotein structure; high performance liquid chromatography; hydroxamate; ionization; mass spectrometry; metalloporphyrins; neuropeptides; neurotoxins; oxidoreductase; peptide structure; phospholipids; polyenes; protein structure; steroids; trehalose; virus protein

The overall objective of the proposed research continues to be to develop and use mass spectrometry as a tool for solving problems of biomedical interest, especially those dealing with the characterization of complex polar compounds and others of high molecular weight. The powerful research mass spectrometers--a VG 70-SE4F and a VG ZAB-SE--will be used in assigning structures to complex peptides and proteins: aldose reductases, cytochromes, marine-derived toxins, insect and marine invertebrate neuropeptides, marine algal and cyanobacterial GABA-mimetic peptides, and peptide antibiotics. The instruments will also be employed in improving or developing the FAB technique: by improving high-resolution FAB; by enhancing ion production with laser assistance; developing LC/HRMS and LC/MS/MS; and by applying LC/FAB to gradient elution HPLC and countercurrent chromatography (CCC). The mass spectrometric techniques developed will be applied to solving structural problems of the P.I. (antiviral and antitumor agents from marine sources, antibiotics, marine-derived chlorins, cyanobacterial toxins, a hibernation factor in bears' blood, hydroxamates from Hemophilus influenzae) and other biomedical scientists at the University of Illinois (i.a., methanogen cofactors, metallo-porphyrins, estrogen receptors, fluorinated steroids, viral proteins, phospholipids, glycolipids, and glycosphingolipids).

拟议研究的总体目标仍然是发展 并使用质谱作为解决生物医学问题的工具 兴趣，特别是那些涉及复杂特征的兴趣 极性化合物和其他高分子量化合物。 功能强大的研究型质谱仪——VG 70-SE4F 和 VG ZAB-SE——将用于指定复杂肽的结构和 蛋白质：醛糖还原酶、细胞色素、海洋毒素、昆虫 和海洋无脊椎动物神经肽、海洋藻类和蓝细菌 GABA 模拟肽和肽抗生素。 这些仪器还将 用于改进或开发 FAB 技术：通过改进 高分辨率FAB；通过激光辅助增强离子产生； 开发 LC/HRMS 和 LC/MS/MS；并应用LC/FAB进行梯度洗脱 HPLC 和逆流色谱 (CCC)。 开发的质谱技术将应用于解决 P.I.的结构性问题（来自海洋的抗病毒和抗肿瘤剂 来源、抗生素、海洋来源的二氢卟酚、蓝藻毒素、 熊血液中的冬眠因子，来自嗜血杆菌的异羟肟酸盐 流感杆菌）和伊利诺伊大学的其他生物医学科学家 （即，产甲烷菌辅因子、金属卟啉、雌激素受体、 氟化类固醇、病毒蛋白、磷脂、糖脂和 鞘糖脂）。