HIGH RESOLUTION MASS SPECTRA OF BIOORGANIC COMPOUNDS

生物有机化合物的高分辨率质谱

• 批准号: 3274483
• 负责人: KENNETH L RINEHART
• 金额: $ 7.68万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-05-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3274483
• 关键词: alkaloids; analytical chemistry; antibacterial agents; antibiotics; antineoplastic antibiotics; antineoplastics; antiviral agents; biomedical automation; biomedical equipment resource; chemical structure; clinical biomedical equipment; clinical chemistry; enzymes; gangliosides; gas chromatography; glycolipids; high performance liquid chromatography; mass spectrometry; neomycin; nucleosides; nucleotides; organic chemicals; peptide structure; pesticide residues; pharmacology; polyenes; porphyrins; pyrophosphates; saltwater environment; toxin metabolism

High resolution mass spectrometry will be employed in studying biomedically important compounds, stressing the new technique of fast atom bombardment mass spectrometry (FABMS). A high field mass spectrometer will be modified to observe ions to m/z 10,000 at maximum accelerating potential (8KV). A variety of ion guns as well as postanalyzer acceleration will be employed to enhance sensitivity. Several matrices will be studied to enhance FAB ion production. Mass markers will be prepared to facilitate computerized data collection and accurate mass measurements of both positive and negative ions, and optimization of high resolution measurements will be studied in ion sources of different designs. Collisional activation will be employed to enhance FAB-induced fragmentation. A high performance liquid chromatograph will be coupled to a FAB mass spectrometer by a modified moving belt apparatus. Compounds to be studied by these techniques include involatile or thermally unstable antibiotics (especially peptides and polyenes), antiviral, antitumor and antimicrobial marine natural products, other peptides (enzymic digests of proteins, fluorogenic peptides, peptide derivatives of drugs and suicide inhibitors), polynucleotides, glycolipids, including gangliosides, coenzymes, terpene pyrophosphates, modified porphyrins, organometallic analogs of metalloenzymes, carcinogenic metabolites, organic compounds with hypervalent atoms, sulfonium salts, and metal carbonyls.

高分辨率质谱分析将用于生物医学研究 重要化合物，强调快原子轰击新技术 质谱（FABMS）。 将改造高场质谱仪 在最大加速电位 (8KV) 下观察 m/z 10,000 的离子。 一个 将采用各种离子枪以及分析仪后加速器 以增强灵敏度。 将研究几个矩阵来增强 FAB 离子的产生。 将准备大量标记以方便计算机化 阳性和阴性的数据收集和精确质量测量 负离子，高分辨率测量的优化将是 研究了不同设计的离子源。 碰撞激活将 用于增强 FAB 诱导的断裂。 高性能 液相色谱仪将通过一个 FAB 质谱仪耦合 改进的移动带装置。 待研究的化合物 技术包括不挥发性或热不稳定的抗生素（特别是 肽和多烯）、抗病毒、抗肿瘤和抗菌海洋 天然产物、其他肽（蛋白质的酶消化物、荧光 肽、药物的肽衍生物和自杀抑制剂）， 多核苷酸、糖脂，包括神经节苷脂、辅酶、萜烯 焦磷酸盐、改性卟啉、有机金属类似物 金属酶、致癌代谢物、有机化合物 高价原子、锍盐和羰基金属。