NEUROBIOLOGY OF DEVELOPMENT

发育的神经生物学

• 批准号: 3100083
• 负责人: WILLIAM T NORTON
• 金额: $ 48.85万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3100083
• 关键词: immunocytochemistry; neurogenesis; neurosciences

This large multidisciplinary program contains seven separate projects devoted to several aspects of nervous system development. Four of the projects are primarily concerned with normal and abnormal glial development, two with cytoskeletal interactions, and one with neutral glycosphingolipids. The major goals are: to further define normal oligodendrocyte lineage and differentiation in vitro; to determine whether acetylCoA- carboxylase is enriched in oligodendroglia and is affected in diabetic rats; to investigate the glial content of glucose-6- phosphate dehydrogenase; to explore oligodendroglial differentiation and development in the myelin-deficient rat mutant, both in vivo and in vitro; to define the origin of astrocytes cultured from mature brain; to explore the nature and biochemistry of reactive astrocytes; to define the composition and enzymology of a large class of reactive astrocytes; to define the composition and enzymology of a large class of neutral glycosphingolipids in whole brain, cell cultures, separate cells, growth cones and sensory neurons; to characterized the immunologic modulation of oligodendrocytes and determine the expression of MHC antigens on these cells; to determine how phosphorylation of neurofilaments affects their assembly, and to explore the nature and biochemistry of interactions of cytoskeletal elements in normal and perturbed nervous system. The methodology includes enzyme purification, preparation of polyvalent and monoclonal antibodies, enzyme assays, immunocytochemistry, tissue culture, (3H)-thymidine labeling, autoradiography, protein phosphorylation, ultrastructure, cytoskeleton purification, protein purification by column chromatography and FPLC, gel electrophoresis, fluorography, filament assembly, microtubule assembly, morphometry, lipid separation and analysis, GLC, TLC, subcellular fractionation, and immunoblotting. The long range goals of this program are to provide detailed information on some well-define processes of differentiation and development of the nervous system, so that we may better understand how they are perturbed by genetic or environmental influences.

这个大型多学科项目包含七个独立的 致力于神经系统多个方面的项目 发展。 其中四个项目主要涉及 正常和异常的神经胶质发育，其中两种具有细胞骨架 相互作用，以及一种与中性糖鞘脂的相互作用。 主要 目标是：进一步定义正常少突胶质细胞谱系和 体外分化；以确定是否乙酰辅酶A- 羧化酶在少突胶质细胞中富集，并受到影响 糖尿病大鼠；研究葡萄糖-6-的神经胶质含量 磷酸脱氢酶；探索少突胶质细胞 髓磷脂缺陷大鼠的分化和发育 体内和体外突变体；来定义原点 从成熟大脑中培养的星形胶质细胞；探索自然和 反应性星形胶质细胞的生物化学；定义组合物 和一大类反应性星形胶质细胞的酶学；定义 一大类中性物质的组成和酶学 全脑、细胞培养物、分离细胞中的鞘糖脂， 生长锥和感觉神经元；来表征 少突胶质细胞的免疫调节并确定 MHC 抗原在这些细胞上的表达；确定如何 神经丝的磷酸化会影响它们的组装，并 探索相互作用的本质和生物化学 正常和紊乱神经系统中的细胞骨架元件。 该方法包括酶纯化、制备 多价和单克隆抗体、酶测定、 免疫细胞化学、组织培养、(3H)-胸苷标记、 放射自显影、蛋白质磷酸化、超微结构、 细胞骨架纯化、蛋白质柱纯化 色谱法和 FPLC、凝胶电泳、荧光照相术、 丝组装、微管组装、形态测定、脂质 分离和分析、GLC、TLC、亚细胞分级分离和 免疫印迹。 该计划的长期目标是提供详细的 有关一些明确定义的分化过程的信息和 神经系统的发育，使我们能够更好地 了解他们如何受到遗传或环境的困扰 影响。