THE CHEMISTRY OF CELLS AND CELL PORTIONS FROM THE CNS

中枢神经系统细胞和细胞部分的化学性质

• 批准号: 3393234
• 负责人: WILLIAM T NORTON
• 金额: $ 45.37万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3393234
• 关键词: astrocytes; cell differentiation; cell free system; central nervous system; chromatography; glia; glycosphingolipids; histochemistry /cytochemistry; immunochemistry; lipids; monoclonal antibody; neurochemistry; neurofilament; neurons; neurotoxins; oligodendroglia; tissue /cell culture

The long term objectives of this proposal are to explore the biochemical properties of intermediate filaments of the nervous system, and the biology of oligodendroglia and astrocytes. The processes of assembly of neurofilament subunits, isolated from rat and bovine CNS, will be studied by turbindimetry and electron microscopy (and new techniques under development). The role of each subunit in this process and the interaction of neurofilaments with other cytoskeletal proteins will be explored by displacement reactions. Neurofilament synthesis will be studied in cell-free systems and metabolism will be studied in normal and toxin-perturbed rats. Long-term cultures of rat oligodendroglia will be characterized and the ability of differentiated oligodendrocytes to divide will be defined. Biochemical studies of oligodendroglia in culture will include protein analysis and biosynthesis, cell-specific enzymes and lipid analyses. Two monoclonal antibodies to oligodendroglia will be further characterized. Attempts to obtain pure cultures of bulk-isolated astrocytes will continue, as will collaborative studies on the glycosphingolipid composition of glial cells. These fundamental studies have implications for conditions such as Alzheimer's disease and toxic neuropathies that involve intermediate filament pathology, and for demyelinating conditions in which stimulation of the possible multiplication of mature oligodendroglia would be desirable.

该提案的长期目标是探索生化 神经系统和生物学的中间细丝的特性 少突胶质和星形胶质细胞。 组装的过程 将研究与大鼠和牛CNS分离的神经丝亚基 通过涡轮辅助和电子显微镜（以及下方的新技术 发展）。 每个亚基在此过程和相互作用中的作用 具有其他细胞骨架蛋白的神经丝的探索 位移反应。 神经丝合成将在 无细胞的系统和代谢将在正常和 毒素扰动的大鼠。 大鼠的长期培养将是 特征和分化的少突胶质细胞分裂的能力 将定义。 培养中少突胶质细胞的生化研究将 包括蛋白质分析和生物合成，细胞特异性酶和脂质 分析。 两种对少突齿的单克隆抗体将进一步 特征。 尝试获得批量分离的纯文化 星形胶质细胞将继续，对 神经胶质细胞的糖磷脂组成。 这些基本研究 对诸如阿尔茨海默氏病和有毒的疾病有影响 涉及中间细丝病理的神经病，用于 脱髓鞘条件，其中可能的刺激 成熟的少突齿的繁殖是可取的。