THE CHEMISTRY OF CELLS AND CELL PORTIONS FROM THE CNS

中枢神经系统细胞和细胞部分的化学性质

• 批准号: 3393237
• 负责人: WILLIAM T NORTON
• 金额: $ 48.36万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3393237
• 关键词: astrocytes; cell differentiation; cell free system; central nervous system; chromatography; glia; glycosphingolipids; histochemistry /cytochemistry; immunochemistry; lipids; monoclonal antibody; neurochemistry; neurofilament; neurons; neurotoxins; oligodendroglia; tissue /cell culture

The long term objectives of this proposal are to explore the biochemical properties of intermediate filaments of the nervous system, and the biology of oligodendroglia and astrocytes. The processes of assembly of neurofilament subunits, isolated from rat and bovine CNS, will be studied by turbindimetry and electron microscopy (and new techniques under development). The role of each subunit in this process and the interaction of neurofilaments with other cytoskeletal proteins will be explored by displacement reactions. Neurofilament synthesis will be studied in cell-free systems and metabolism will be studied in normal and toxin-perturbed rats. Long-term cultures of rat oligodendroglia will be characterized and the ability of differentiated oligodendrocytes to divide will be defined. Biochemical studies of oligodendroglia in culture will include protein analysis and biosynthesis, cell-specific enzymes and lipid analyses. Two monoclonal antibodies to oligodendroglia will be further characterized. Attempts to obtain pure cultures of bulk-isolated astrocytes will continue, as will collaborative studies on the glycosphingolipid composition of glial cells. These fundamental studies have implications for conditions such as Alzheimer's disease and toxic neuropathies that involve intermediate filament pathology, and for demyelinating conditions in which stimulation of the possible multiplication of mature oligodendroglia would be desirable.

该提案的长期目标是探索生化 神经系统中间丝的特性和生物学 少突胶质细胞和星形胶质细胞。 的组装过程 将研究从大鼠和牛中枢神经系统中分离出的神经丝亚基 通过比浊法和电子显微镜（以及新技术 发展）。 各亚基在此过程中的作用及相互作用 神经丝与其他细胞骨架蛋白的结合将被探索 置换反应。 神经丝合成将在 无细胞系统和新陈代谢将在正常和 受毒素干扰的老鼠。 大鼠少突神经胶质细胞的长期培养 分化少突胶质细胞的特征和分裂能力 将被定义。 培养中少突胶质细胞的生化研究将 包括蛋白质分析和生物合成、细胞特异性酶和脂质 分析。 两种针对少突胶质细胞的单克隆抗体将进一步 特点。 尝试获得批量分离的纯培养物 星形胶质细胞将继续下去，关于星形胶质细胞的合作研究也将继续 神经胶质细胞的鞘糖脂成分。 这些基础研究 对阿尔茨海默病和中毒等疾病有影响 涉及中间丝病理学的神经病，以及 脱髓鞘条件，其中可能的刺激 成熟少突胶质细胞的增殖将是理想的。