Characterisation of vital carbohydrate synthases in pathogenic oomycetes

致病卵菌中重要碳水化合物合酶的表征

• 批准号: DP180103974
• 负责人: Prof Vincent Bulone
• 金额: $ 38.26万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-01-01 至 2021-10-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP180103974
• 关键词: Characterisation; vital; carbohydrate; synthases; pathogenic

This project aims to understand the mechanisms that control cell wall stability in the fish pathogen, Saprolegnia parasitica. The biochemical properties and function of vital enzymes involved in cell wall biosynthesis will be determined using innovative approaches at the interface of biochemistry, microbiology, cell biology, and structural biology. Next generation ion mobility mass spectrometry will be used to solve challenging structural questions that cannot be tackled with conventional techniques. Expected outcomes include new knowledge on challenging membrane proteins that allows development of novel strategies for disease control in aquaculture. The data may also be applicable to crop protection from related plant pathogens.

该项目旨在了解鱼类病原体寄生水霉中控制细胞壁稳定性的机制。参与细胞壁生物合成的重要酶的生化特性和功能将利用生物化学、微生物学、细胞生物学和结构生物学交叉领域的创新方法来确定。下一代离子淌度质谱仪将用于解决传统技术无法解决的具有挑战性的结构问题。预期成果包括有关具有挑战性的膜蛋白的新知识，从而可以开发水产养殖疾病控制的新策略。该数据也可能适用于作物保护免受相关植物病原体的侵害。