CENTRAL TRIGEMINAL PLASTICITY--EFFECT OF SENSORY ORGAN 3

中央三叉神经可塑性--感觉器官3的作用

• 批准号: 3088562
• 负责人: DAVID A SIROIS
• 金额: $ 5.64万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088562
• 关键词: afferent nerve; autoradiography; denervation; deoxyglucose; neural information processing; neural plasticity; peripheral nervous system; sensorimotor system

This study will examine aspects of neuronal plasticity in the developing and mature central nervous system (CNS) involved in the functional (metabolic) reorganization of pre-existing neural connections in response to receptor organ damage. Central plasticity is especially relevant to such issues as recovery of cortical function following partial loss of sensory input, the pathogenesis of congenital CNS malformations, skill acquisition, and brain information storage processes in the normal animal. These experiments will extend our knowledge of the means by which to evaluate the effect of treatment on improving functional recovery as well as provide the evidence needed to determine the level of recovery. First, using a labeled analogue of glucose, 2-deoxy-D-(14C) glucose, (2DG method) injected into a naive (unaltered) rat, the normal pattern of metabolic (functional) activity in the afferent pathway between peripheral receptor and cortical representation will be analyzed through quantitative autoradiography following C3 vibrissa stimulation. Second, utilizing the same labeling technique, a separate group of rats will be examined following peripheral receptor organ damage (ablation of all facial vibrissa follicles except C3-spared C3 model). The spared C3 vibrissa will then be stimulated and the 2DG labeling analyzed in order to examine the effect of peripheral sensory denervation on functional reorganization at subcortical levels in the precisely organized rat facial vibrissal-cortical "barrel" system. Third, light microscopic anatomical changes associated with the functional reorganization in the deafferented rat CNS will be examined, aided by the precise metabolic map produced by the autoradiographic 2DG technique. Finally, the relationship between the stage of CNS development and the extent and pattern of subcortical functional reorganization will be studied in the vibrissa-deafferented rat.

这项研究将检查神经元可塑性的各个方面 发育和成熟的中枢神经系统（CNS）参与 预先存在的神经的功能（代谢）重组 响应受体器官损伤的连接。 中央 可塑性与诸如恢复等问题尤其相关 部分感觉输入丧失后的皮质功能 先天性中枢神经系统畸形的发病机制、技能习得、 和正常动物的大脑信息存储过程。 这些实验将扩展我们对这些方法的了解 用于评估治疗对改善功能的效果 恢复并提供确定所需的证据 恢复水平。 首先，使用标记的葡萄糖类似物 2-脱氧-D-(14C) 将葡萄糖（2DG 方法）注射到幼稚（未改变的）大鼠体内， 传入神经代谢（功能）活动的正常模式 外周受体和皮质表征之间的通路 将通过以下定量放射自显影进行分析 C3 触毛刺激。 二、使用相同的标签 技术，将在以下情况下检查一组单独的大鼠 外周感受器官损伤（所有面部触毛消融） 卵泡（C3 保留的 C3 模型除外）。 幸存的 C3 触须将 然后受到刺激并分析 2DG 标记，以便 检查周围感觉去神经支配的影响 皮层下水平的功能重组精确地 有组织的大鼠面部触毛皮质“桶”系统。 第三， 与光显微解剖学相关的变化 大鼠中枢神经系统的功能重组 在精确的代谢图的帮助下进行检查 放射自显影 2DG 技术。 最后，关系 中枢神经系统发育的阶段和程度之间 将研究皮层下功能重组的模式 触须失聪的老鼠