CENTRAL TRIGEMINAL PLASTICITY: EFFECT OF SENSORY ORGAN

中央三叉神经可塑性：感觉器官的影响

• 批准号: 3088564
• 负责人: DAVID A SIROIS
• 金额: $ 6.07万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088564
• 关键词: afferent nerve; autoradiography; denervation; deoxyglucose; developmental neurobiology; laboratory rat; neural information processing; neural plasticity; peripheral nervous system; sensorimotor system; trigeminal nerve

This study will examine aspects of neuronal plasticity in the developing and mature central nervous system (CNS) involved in the functional (metabolic) reorganization of pre-existing neural connections in response to receptor organ damage. Central plasticity is especially relevant to such issues as recovery of cortical function following partial loss of sensory input, the pathogenesis of congenital CNS malformations, skill acquisition, and brain information storage processes in the normal animal. These experiments will extend our knowledge of the means by which to evaluate the effect of treatment on improving functional recovery as well as provide the evidence needed to determine the level of recovery. First, using a labeled analogue of glucose, 2-deoxy-D-(14C) glucose, (2DG method) injected into a naive (unaltered) rat, the normal pattern of metabolic (functional) activity in the afferent pathway between peripheral receptor and cortical representation will be analyzed through quantitative autoradiography following C3 vibrissa stimulation. Second, utilizing the same labeling technique, a separate group of rats will be examined following peripheral receptor organ damage (ablation of all facial vibrissa follicles except C3-spared C3 model). The spared C3 vibrissa will then be stimulated and the 2DG labeling analyzed in order to examine the effect of peripheral sensory denervation on functional reorganization at subcortical levels in the precisely organized rat facial vibrissal-cortical "barrel" system. Third, light microscopic anatomical changes associated with the functional reorganization in the deafferented rat CNS will be examined, aided by the precise metabolic map produced by the autoradiographic 2DG technique. Finally, the relationship between the stage of CNS development and the extent and pattern of subcortical functional reorganization will be studied in the vibrissa-deafferented rat.

这项研究将检查神经元可塑性的各个方面 涉及的发展和成熟的中枢神经系统（CNS） 现有神经的功能性（代谢）重组 响应受体器官损伤的连接。 中央 可塑性与恢复等问题特别相关 部分丧失感觉输入后的皮质功能， 先天性中枢神经系统畸形的发病机理，技能获取， 正常动物中的大脑信息存储过程。 这些实验将通过 评估治疗对提高功能的影响 恢复以及提供确定所需的证据 恢复水平。 首先，使用葡萄糖的标记类似物2-脱氧-D-（14C） 葡萄糖（2DG方法）注入幼稚（未改变）大鼠， 传入中代谢（功能）活性的正常模式 外围受体和皮质表示之间的途径 将通过定量放射自显影来分析 C3颤音刺激。 第二，利用相同的标签 技术，将检查一组单独的大鼠 外围受体器官损伤（所有面部颤音的消融 除了C3比较C3模型以外）。 不幸的C3 Vibrissa将 然后被刺激并分析2DG标记，以便 检查周围感觉神经神经的影响 精确的皮层下水平的功能重组 有组织的大鼠面部振动皮层“枪管”系统。 第三， 光学显微镜解剖学变化与 脱离的大鼠中枢神经系统中的功能重组将是 检查，由由 放射自显影2DG技术。 最后，关系 在中枢神经系统发展阶段与程度和程度和 将研究皮层功能重组的模式 Vibrissa偏鼠的大鼠。