CORE--BIOPHYSICAL AND BIOCHEMICAL FACILITIES

核心--生物物理和生化设施

• 批准号: 6235878
• 负责人: RENNE C LU
• 金额: $ 17.06万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-15 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6235878
• 关键词: biochemistry; biomedical equipment resource; biomedical facility; biophysics; peptide chemical synthesis; protein purification

Core B provides technical support for all projects. It includes three parts: (1) Protein Chemistry: provides amino acid composition and sequence analyses, and synthesis of peptides using various state-of-the-art facilities. It will also offer advice to individual PI's on peptide/fragment purification and characterization. This service will be used essentially by every project, with less emphasis in Projects II-C and III. (2) Analytical Ultracentrifugation: provides both sedimentation velocity and sedimentation equilibrium runs, as well as related hydrodynamic analyses; it will support Projects I-A, I-B, II-A and II-B. (3) Electron Microscopy and Immunocytochemistry: provides technical expertise and advice for the EM visualization of proteins, especially with an improved rotary shadowing technique recently developed by one of the PI's, and supports the preparation of polyclonal and monoclonal antibodies, including the handling of laboratory animals. This service will be needed in Projects I-A, II-A, II-B and possibly I-B and III.

Core B为所有项目提供技术支持。 它包括三个 部分： (1) 蛋白质化学：提供氨基酸组成和 使用各种最先进的技术进行序列分析和肽合成 设施。 它还将为个人 PI 提供以下方面的建议： 肽/片段纯化和表征。 这项服务将 基本上每个项目都使用，较少强调项目 II-C 和 三． (2) 分析超速离心：提供沉淀 速度和沉降平衡运行，以及相关的 水动力分析；它将支持项目 I-A、I-B、II-A 和 II-B。 (3) 电子显微镜和免疫细胞化学：提供技术 蛋白质 EM 可视化的专业知识和建议，尤其是 最近由其中一家公司开发的一种改进的旋转阴影技术 PI，并支持多克隆和单克隆抗体的制备， 包括实验动物的处理。 将需要这项服务 在项目 I-A、II-A、II-B 以及可能的 I-B 和 III 中。