CORE--BIOPHYSICAL AND BIOCHEMICAL FACILITIES

核心--生物物理和生化设施

• 批准号: 6571150
• 负责人: RENNE C LU
• 金额: $ 27.63万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6571150
• 关键词: aminoacid analyzer; analytical ultracentrifugation; biomedical equipment resource; biomedical facility; electron microscopy; immunocytochemistry; mass spectrometry; peptide chemical synthesis; protein purification

Description: This core will consist of a Protein Chemistry facility under Dr. Lu, an Analytical Ultracentrifugation facility under Walter. F. Stafford, Ph.D., and an Electron Microscopy and Immunocytochemistry facility under Katsuhide Mabuchi, Ph.D. The Protein Chemistry part of the Core will provide services for all projects in making synthetic peptides and analyzing peptides/proteins for amino acid compositions and/or sequences. It will also provide advice concerning peptides/proteins purification and characterization. The Analytical Ultracentrifugation part of the Core will primarily support Projects 1, 2-A, 2-B and 3. This service provides both sedimentation velocity and sedimentation equilibrium analysis, as well as related hydrodynamic analyses. The third part of Core B will provide services with the combined use of electron microscopy and immunocytochemistry. It offers technical expertise and advice for the EM visualization of proteins, especially with an improved rotary shadowing technique developed by the PI, and supports the preparation of polyclonal and monoclonal antibodies, including the handling of laboratory animals.

描述： 该核心将包括卢博士领导下的蛋白质化学设施， 瓦尔特 (Walter) 旗下的分析超速离心设施。 F.斯塔福德博士，和 Katsuhide 领导下的电子显微镜和免疫细胞化学设施 万渊博士 核心的蛋白质化学部分将为所有项目提供服务 制造合成肽和分析肽/蛋白质的氨基酸 组合物和/或序列。它还将提供有关以下方面的建议 肽/蛋白质纯化和表征。 核心的分析超速离心部分将主要支持 项目 1、2-A、2-B 和 3。该服务提供沉降速度 和沉降平衡分析，以及相关的水动力 分析。 Core B的第三部分将结合使用提供服务 电子显微镜和免疫细胞化学。它提供技术专业知识和 对于蛋白质的 EM 可视化的建议，特别是改进的 PI开发的旋转阴影技术，并支持制备 多克隆和单克隆抗体，包括实验室的处理 动物。