Procise Protein Sequencing System

Procise蛋白质测序系统

• 批准号: 6440940
• 负责人: RENNE C LU
• 金额: $ 14.66万
• 依托单位: BOSTON BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440940
• 关键词: biomedical equipment purchase; protein protein interaction; protein sequence; protein structure function

DESCRIPTION (provided by applicant): A user group consisting of twenty principal investigators and their research staff at Boston Biomedical Research Institute, working on more than twenty NIH-funded projects, is requesting funds for the purchase of a Procise Protein Sequencing System from Applied Biosystems. These investigators are conducting a wide range of research projects, which focus on topics such as protein-protein interactions and structure-function relationships of proteins in contractile systems and heat shock proteins, subunit interactions in membrane-associated energy transduction and transport systems, mechanisms of cell death and signaling pathways, the design of immunotherapeutic agents to treat Alzheimer disease, and the crystal structures of various proteins. The requested instrument is to replace a 12-year old ABI 477, which has recently become nonfunctional. An in-house sequencing facility is needed for the verification of protein and protein fragments generated by recombinant DNA techniques and the assessment of protein fragments for crystallographic studies. N-terminal sequencing in conjunction with mass spectrometry is also essential for studies involving identification of binding partners, determination of epitopes, identification of post-translational modification sites and determination of proximity relationships within or between proteins. The requested instrument will be housed in the Institute's Protein/Peptide Core Facility and will be supervised by Dr. Renne Lu, the director of the Core Facility. Dr. Lu and Ms. Ming-Jen Tsay will be the designated operators and responsible for the maintenance of the instrument. It is anticipated that research activities at BBRI will keep the instrument well utilized. However, scientists from other research institutions will also be given access to this instrument on an availability basis.

描述（由申请人提供）：一个由二十人组成的用户组 波士顿生物医学研究中心的主要研究人员及其研究人员 该研究所致力于 20 多个 NIH 资助的项目，正在申请资金 从 Applied 公司购买 Procise 蛋白质测序系统 生物系统。这些研究人员正在进行广泛的研究 项目，重点关注蛋白质-蛋白质相互作用等主题 收缩系统和热量中蛋白质的结构-功能关系 休克蛋白，膜相关能量转导中的亚基相互作用 和运输系统、细胞死亡机制和信号通路、 治疗阿尔茨海默病的免疫治疗剂的设计及其晶体 各种蛋白质的结构。 所请求的仪器是为了替换已有 12 年历史的 ABI 477，该仪器已使用 最近变得不起作用。需要内部测序设施 重组 DNA 生成的蛋白质和蛋白质片段的验证 晶体学技术和蛋白质片段评估 研究。 N 端测序与质谱联用也 对于涉及识别结合伙伴的研究至关重要， 表位测定、翻译后修饰鉴定 蛋白质内部或蛋白质之间的邻近关系的位点和确定。 所需的仪器将安装在研究所的蛋白质/肽核心中 设施并将由核心主任 Renne Lu 博士监督 设施。陆博士和蔡明仁女士将担任指定操作员 负责仪器的维护保养。预计 BBRI 的研究活动将使该仪器得到充分利用。然而， 其他研究机构的科学家也将有机会获得这一成果 仪器以可用性为基础。