METABOLIC ADAPTATIONS TO REPETITIVE MYOCARDIAL ISCHEMIA

重复性心肌缺血的代谢适应

• 批准号: 2879389
• 负责人: David G Van Wylen
• 金额: $ 10.59万
• 依托单位: ST. OLAF COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2879389
• 关键词: adenosine; adenosine deaminase; adenosine kinase; cytoprotection; interstitial; laboratory rabbit; microdialysis; myocardial ischemia /hypoxia; purine /pyrimidine metabolism; reperfusion

DESCRIPTION: (Adapted from the Investigator's Abstract) Preconditioning induced by repeated brief myocardial ischemia each separated by another brief period of reperfusion protects the myocardium from subsequent ischemia reperfusion injury. One of beneficial consequences of preconditioning is progressive preservation of high energy phosphate compounds which is reflected by the progressively greater attenuation of the increase in interstitial fluid (ISF) purine metabolites. The proposed research will examine the threshold of the attenuated purine metabolite accumulation by addressing two Specific Aims: 1) by determining the minimum time and degree of ischemia necessary to induce attenuated purine metabolite accumulation during subsequent global ischemia and 2) by determining the effect of adenosine kinase or adenosine deaminase inhibition on ISF purine metabolite accumulation and by studying the rate of adenosine formation during preconditioning.

描述：（改编自研究者的摘要）诱导的预处理 重复短暂的心肌缺血，每次间隔另一个短暂的时间 再灌注可保护心肌免受随后的缺血再灌注 受伤。预处理的有益后果之一是渐进的 高能磷酸化合物的保存，这反映在 间质液 (ISF) 增加的逐渐减弱 嘌呤代谢物。拟议的研究将检查阈值 通过解决两个具体目标来减少嘌呤代谢物的积累：1) 通过确定诱导所需的最短缺血时间和程度 随后的全身缺血期间嘌呤代谢物的积累减弱， 2)通过测定腺苷激酶或腺苷脱氨酶的作用 通过研究 ISF 嘌呤代谢物积累的抑制率 预处理过程中腺苷的形成。