CARDIAC MICRODIALYSIS, ADENOSINE, AND CARDIAC FUNCTION

心脏微透析、腺苷和心脏功能

• 批准号: 3365173
• 负责人: David G Van Wylen
• 金额: $ 13.5万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3365173
• 关键词: Mammalia; adenosine; heart circulation; heart function; heart metabolism; heart pharmacology; histology; hypoxanthines; inosine; interstitial; lactates; microdialysis; myocardial ischemia /hypoxia; xanthines

DESCRIPTION (adapted from applicant's abstract): The objectives of the proposed research are: 1) to explore the usefulness of the cardiac microdialysis technique for the sampling of intramyocardial interstitial fluid (ISF) and measurement of regional coronary blood flow (CBF); and 2) to use the cardiac microdialysis technique to study the cardiac effects of adenosine. In the proposed experiments, cardiac microdialysis, used to determine ISF levels of adenosine, inosine, hypoxanthine, xanthine, uric acid, and lactate, would be utilized in conjunction with standard techniques for the measurement of regional ventricular function and metabolism to perform four series of experiments. Series 1 would assess the validity, applicability, and limitations of the cardiac microdialysis technique. The experiments of Series 2 would take advantage of the capability of the cardiac microdialysis technique to deliver pharmacological agents locally to the tissue surrounding the microdialysis probe, using this approach to study adenosine metabolism and action. In Series 3, cardiac microdialysis would be used to investigate changes in regional ISF purine metabolites and lactate would be correlated to changes in local CBF and regional ventricular function during hypoxia, inotropic stimulation, and graded reductions in coronary artery perfusion pressure. In Series 4, cardiac microdialysis would be used to investigate myocardial ischemia, focusing on interventions which alter endogenous adenosine levels during ischemia.

描述（改编自申请人的摘要）：该项目的目标 拟议的研究是：1）探索心脏的有用性 用于心肌内间质取样的微透析技术 液体（ISF）和局部冠状动脉血流（CBF）测量；和 2) 使用心脏微透析技术研究心脏作用 腺苷。 在拟议的实验中，心脏微透析用于 测定腺苷、肌苷、次黄嘌呤、黄嘌呤、尿酸的 ISF 水平 酸和乳酸盐，将与标准一起使用 测量局部心室功能的技术和 新陈代谢进行四个系列的实验。 系列 1 将评估 心脏微透析的有效性、适用性和局限性 技术。 系列 2 的实验将利用 心脏微透析技术的能力 局部作用于微透析周围组织的药物 探针，使用这种方法来研究腺苷代谢和作用。 在 系列 3，心脏微透析将用于研究 区域 ISF 嘌呤代谢物和乳酸的变化与相关 缺氧、正性肌力期间局部 CBF 和局部心室功能 刺激和冠状动脉灌注压的分级降低。 在系列 4 中，心脏微透析将用于研究心肌 缺血，重点关注改变内源性腺苷水平的干预措施 缺血期间。