CARDIAC MICRODIALYSIS, ADENOSINE, AND CARDIAC FUNCTION

心脏微透析、腺苷和心脏功能

• 批准号: 3365172
• 负责人: David G Van Wylen
• 金额: $ 13.42万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3365172
• 关键词: Mammalia; adenosine; heart circulation; heart function; heart metabolism; heart pharmacology; histology; hypoxanthines; inosine; interstitial; lactates; microdialysis; myocardial ischemia /hypoxia; xanthines

DESCRIPTION (adapted from applicant's abstract): The objectives of the proposed research are: 1) to explore the usefulness of the cardiac microdialysis technique for the sampling of intramyocardial interstitial fluid (ISF) and measurement of regional coronary blood flow (CBF); and 2) to use the cardiac microdialysis technique to study the cardiac effects of adenosine. In the proposed experiments, cardiac microdialysis, used to determine ISF levels of adenosine, inosine, hypoxanthine, xanthine, uric acid, and lactate, would be utilized in conjunction with standard techniques for the measurement of regional ventricular function and metabolism to perform four series of experiments. Series 1 would assess the validity, applicability, and limitations of the cardiac microdialysis technique. The experiments of Series 2 would take advantage of the capability of the cardiac microdialysis technique to deliver pharmacological agents locally to the tissue surrounding the microdialysis probe, using this approach to study adenosine metabolism and action. In Series 3, cardiac microdialysis would be used to investigate changes in regional ISF purine metabolites and lactate would be correlated to changes in local CBF and regional ventricular function during hypoxia, inotropic stimulation, and graded reductions in coronary artery perfusion pressure. In Series 4, cardiac microdialysis would be used to investigate myocardial ischemia, focusing on interventions which alter endogenous adenosine levels during ischemia.

描述（改编自申请人的摘要）： 拟议的研究是：1）探索心脏的有用性 微透析技术，用于对心脏内部心肌的采样 液体（ISF）和区域冠状动脉血流（CBF）的测量；和2） 使用心脏微透析技术来研究 腺苷。 在拟议的实验中，心脏微透析过去 确定ISF水平的腺苷，肌苷，低黄嘌呤，黄嘌呤，尿液 酸和乳酸将与标准结合使用 测量区域心室功能的技术和 代谢进行四个系列实验。 系列1将评估 心脏微透析的有效性，适用性和局限性 技术。 系列2的实验将利用 心脏微透析技术的能力传递 在微透析周围的组织本地的药理学剂 探测，使用这种方法研究腺苷代谢和作用。 在 系列3，心脏微透析将用于研究 区域ISF嘌呤代谢物和乳酸将与变化相关 在缺氧期间局部CBF和区域心室功能中 刺激和冠状动脉灌注压力的分级减少。 在第4系中，心脏微透析将用于研究心肌 缺血，重点是改变内源性腺苷水平的干预措施 在缺血期间。