EMOTION OF PAIN AND ITS SUPPRESSION

痛苦的情绪及其抑制

• 批准号: 2635704
• 负责人: GEORGE S. BORSZCZ
• 金额: $ 10.95万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2635704
• 关键词: behavioral /social science research tag; brain; emotions; laboratory rat; morphine; naloxone; neuroanatomy; neuropharmacology; pain; vocalization

The applicant suggests that pain experience includes: (1) the sensory- discriminative dimension that signals the location, intensity and physical properties of a noxious stimulus and evokes rapid responses designed to prevent potential or future injury; (2) the affective-motivational dimension that ascribes to a noxious stimulus the affective-motivational dimension that ascribes to a noxious stimulus the percept of unpleasantness that motivates behaviors such as avoidance and recuperation; and (3) a cognitive-evaluative dimension that induces an appraisal of the meanings and consequences associated with painful sensations and injury. The current application is a second revision of a renewal after a FIRST award to the investigator. The focus of this application is the affective- motivational dimension of the pain experience with vocalization after discharges (VADs; vocalizations of the rat that exceed the application of the noxious experience; e.g. tailshock) as a model for this dimension. In previous studies by the investigator and other investigators, the investigator has concluded that VADs are elicited from a reciprocal rhinencephalic-diencephalic circuit which includes the vPAG/DR, nucleus parafascicularis, amygdala, habenula, and nucleus accumbens. The general hypothesis is that morphine, acting within the vPAG/DR, attenuate VADs by activating serotonergic neurons which project to the re=levant limbic areas; serotonergic neurons, in turn, activate enkephalinergic neurons, which contribute to the suppression of VADs. The proposed studies are designed (1) to examine the contribution of each of these brain areas to the elicitation of VADs and to the ability of vPAG/DR morphine to attenuate VADs; 92) to test the hypothesis that serotonergic neurons contact enkephalinergic neurons in each of the limbic areas; and (3) examine the hypothesis that morphine-induced attenuation of VADs requires the cooperation of each of the brain areas (e.g. removal of even one of the areas will reduce the effectiveness of morphine). The overall strategy is to examine the ability of the 5-HT antagonist methysergide and the opioid antagonist naloxone to antagonize the effects of morphine in the vPAG/DR. Each site will be examined in combination with morphine in the vPAG/DR and each site will be examined for the combination of methysergide and naloxone to test hypothesis (2) above The five anatomical sites will then be examined in pairs to test hypothesis (3) above. Each brain area will be subjected to electrical stimulation and chemical (NMDA) stimulation to test the hypothesis that activation of this area elicits VAD-like responses and each brain area will be damaged to test the hypothesis that removal of this area reduces tailshock elicited VADs.

申请人建议疼痛经历包括：（1）感觉 - 判别性维度标志着位置，强度和物理 有害刺激的特性，并唤起旨在的快速反应 防止潜在或未来的伤害； （2）情感动机 归因于情感动机的有害刺激的维度 归因于有害刺激的维度 这激发了诸如避免和恢复的行为； （3）a 认知评估维度，导致对含义的评估 以及与痛苦的感觉和伤害有关的后果。 这 当前申请是续订的第二次修订 致调查员。 该应用的重点是情感 - 发声之后的痛苦体验的动机维度 排放（VADS；超过应用的老鼠发声 有害的经历；例如尾声）作为此维度的模型。 在研究人员和其他研究人员的先前研究中 研究者得出的结论是，vad是从倒数中引起的 鼻脑功能电路，包括VPAG/DR，Nucleus 旁皮，杏仁核，Habenula和伏隔核。 将军 假设是吗啡作用在VPAG/DR内，通过 激活5-左右左右的5-羟色源性神经元 区域；血清素能神经元反过来激活脑脑能神经元， 这有助于抑制VAD。 拟议的研究是 设计（1）来检查这些大脑区域对 VADS的启发和VPAG/DR吗啡的能力减弱 vads; 92）检验5-羟色源性神经元接触的假设 在每个边缘区域中的脑脑能神经元； （3）检查 吗啡诱导的VAD衰减的假设需要 每个大脑区域的合作 区域将降低吗啡的有效性）。 总体策略是检查5-HT拮抗剂的能力 甲基甲基和阿片类药物拮抗剂纳洛酮拮抗作用 VPAG/DR中的吗啡。 每个站点将与 VPAG/DR中的吗啡和每个站点的组合将检查 甲基甲基和纳洛酮以在五个上方检验假设（2） 然后，将成对检查解剖位点以检验假设（3） 多于。 每个大脑区域都会受到电刺激和 化学（NMDA）刺激以检验激活的假设 区域引起类似VAD的响应，每个大脑区域都会损坏进行测试 去除该区域的假设减少了尾骨的引起vads。