Microscope system for large scale optical imaging of neuronal activity using kilohertz frame rates

使用千赫兹帧速率对神经元活动进行大规模光学成像的显微镜系统

基本信息

批准号：
10384932
负责人：
JACOB R GLASER
金额：
$ 99.53万
依托单位：
MICROBRIGHTFIELD, LLC
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-01-01 至 2022-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10384932
关键词：
Address Behavioral Biological Biosensor Biotechnology Brain Brain Diseases Calcium Cells Central Nervous System Diseases Cerebral cortex Collaborations Communities Complex Computer software Cortical Column Development Devices Electrodes Feasibility Studies Fiber Functional Magnetic Resonance Imaging Generations Human Image In Vitro Individual Institutes Interest Group Knowledge Laser Scanning Microscopy Lasers Letters Light Medical Microscope Morphologic artifacts Motion Mus Neuraxis Neurodegenerative Disorders Neurodevelopmental Disorder Neuromodulator Neurons Neurosciences Research Neurotransmitters Optics Pattern Phase Photometry Population Process Production Research Resolution Scanning Signal Transduction Slice Societies Specimen Stimulus Surface System Technology Testing Time Validation Wisconsin Work base design digital improved in vivo innovation interest invention millisecond neuropsychiatric disorder new technology next generation novel novel therapeutics optical imaging prevent prototype research and development temporal measurement tomography treatment strategy two-photon usability voltage

项目摘要

Abstract This project aims to develop the 2P-ActivityScope™, a revolutionary new microscope based on a technological breakthrough called second-generation Scanned Line Angular Projection (SLAP2) two photon laser scanning microscopy that was recently developed by Dr. Kaspar Podgorski (Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA). The game-changing innovation in the 2P-ActivityScope is the ability to perform unparalleled (i) optical imaging of neuronal activity in populations of neurons at subcellular spatial resolution and temporal resolution in the millisecond range both in vivo and in vitro using a variety of fluorescent indicators (e.g. voltage, [neurotransmitter]) as well as (ii) high-resolution volumetric calcium imaging of columns of cortical neurons at 40 Hz in vivo. These capabilities are crucial for ultimately decoding how information is represented and processed by the billions of densely interconnected neurons comprising the mammalian central nervous system. Neither contemporary 2-photon microscopes, functional magnetic resonance imaging, inserting electrodes into the brain, or fiber photometry can serve this need. This project will disseminate and improve upon the original, lab-built SLAP2 invention to create a commercial product for this important new technology. Based on pilot work performed at Janelia, it is clear that the 2P-ActivityScope will make a significant impact on the field of neuroscience research, including advancing studies focused on alterations in CNS circuitry associated with neurodevelopmental, neuropsychiatric and neurodegenerative disorders. Ultimately, this will result in an improved basis for developing novel treatment strategies for a wide spectrum of complex brain diseases. In Phase I we will demonstrate feasibility of this novel technology by developing prototype hardware and software; work in Phase II will focus on creating the full functionality of the 2P-ActivityScope for commercial release. We will perform extensive feasibility studies, product validation and usability studies of the 2P-ActivityScope in close collaboration with Dr. Podgorski and our academic collaboration partners. A competing technology is not commercially available.

抽象的该项目旨在开发 2P-ActivityScope™，这是一款基于技术的革命性新型显微镜突破称为第二代扫描线角投影（SLAP2）双光子激光扫描最近由 Kaspar Podgorski 博士（Howard Hughes 珍妮莉亚研究园区）开发的显微镜医学研究所，阿什本，弗吉尼亚州）。2P-ActivityScope 的颠覆性创新在于执行能力。无与伦比的 (i) 以亚细胞空间分辨率对神经元群体中的神经元活动进行光学成像使用各种荧光指示剂（例如，体内和体外）的时间分辨率在毫秒范围内电压，[神经递质]）以及（ii）皮质柱的高分辨率体积钙成像体内 40 Hz 的神经元这些功能对于最终解码信息的表示方式至关重要。并由构成哺乳动物中枢神经系统的数十亿个紧密互连的神经元处理当代的 2 光子显微镜、功能性磁共振成像、插入系统都不是。进入大脑的电极或光纤光度测量可以满足这一需求。最初的实验室构建的 SLAP2 发明旨在为这项重要的新技术创建商业产品。根据 Janelia 进行的试点工作，很明显 2P-ActivityScope 将对该领域产生重大影响神经科学研究，包括重点关注中枢神经系统回路改变的进展研究最终，这将导致神经发育、神经精神和神经退行性疾病。为开发针对广泛的复杂脑部疾病的新治疗策略奠定了基础。第一阶段，我们将通过开发原型硬件和软件来证明这项新技术的可行性；第二阶段的工作将侧重于创建 2P-ActivityScope 的完整功能以供商业发布。将密切对 2P-ActivityScope 进行广泛的可行性研究、产品验证和可用性研究与 Podgorski 博士和我们的学术合作伙伴的合作不是竞争性技术。市售。