Viral Testing Core

病毒检测核心

• 批准号: 10373934
• 负责人: Gregory A Dissen
• 金额: $ 4.52万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10373934
• 关键词: AIDS-Related Opportunistic Infections; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Address; Algorithms; Animal Model; Animals; Biological Assay; Biological Models; Breeding; Cost Savings; Custom; Derivation procedure; Development; Disease; Ensure; Enzyme-Linked Immunosorbent Assay; Funding; Genetic; Goals; HIV; Health; Individual; Macaca mulatta; Maintenance; Microarray Analysis; Monitor; Oregon; Pathogenesis; Primates; Protein Microarray Assay; Protein Microchips; Regimen; Reproducibility; Research; Resources; Sensitivity and Specificity; Serology test; Standardization; Testing; Time; United States National Institutes of Health; Vaccines; Validation; Viral; Viral Antibodies; Virus; Zoonoses; germ free condition; improved; next generation; nonhuman primate; pathogen; pathogenic virus; screening; testing services

PROJECT SUMMARY, Core B: Viral Testing Core Genetically characterized rhesus macaques that are free of endemic viral pathogens have become the premier animal model for HIV/AIDS research, including studies on HIV pathogenesis, AIDS-related opportunistic infections, as well as the development of HIV treatments and vaccines. The establishment of specific pathogen-free (SPF) colonies also serves to protect animal caretakers from zoonotic diseases and improves overall animal health. However, the successful derivation and maintenance of SPF colonies is challenging and requires strict surveillance regimens over long periods of time. Therefore, the overall aim of the Viral Testing Core is to provide appropriate pathogen surveillance and implement state-of-the-art testing services, in order to maintain our SPF colonies free of disease. To do this, we will use proven virus screening algorithms combined with an expanding arsenal of screening assays. A central strategy to achieve this will be the implementation of a new multiplexed protein microarray assay platform for routine colony screening, which we have developed and validated at our center.

项目摘要，核心 B：病毒检测核心 不含地方性病毒病原体的基因特征恒河猴已成为首选 用于艾滋病毒/艾滋病研究的动物模型，包括艾滋病毒发病机制、艾滋病相关机会性研究 感染，以及艾滋病毒治疗和疫苗的开发。设立具体 无病原体（SPF）菌落还可以保护动物饲养员免受人畜共患疾病的侵害，并改善 动物整体健康状况。然而，SPF 菌落的成功衍生和维持具有挑战性，并且 需要长期严格的监测方案。因此，病毒检测的总体目标 核心是提供适当的病原体监测并实施最先进的检测服务，以便 保持我们的 SPF 菌落免受疾病侵害。为此，我们将结合使用经过验证的病毒筛查算法 不断扩大的筛选试验库。实现这一目标的核心战略是实施 我们开发了一种用于常规菌落筛选的新型多重蛋白质微阵列分析平台 并在我们中心进行了验证。