Component: Husbandry and Management Core

组成部分：畜牧和管理核心

• 批准号: 10338458
• 负责人: Kamm Prongay
• 金额: $ 141.29万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10338458
• 关键词: AIDS-Related Opportunistic Infections; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Animal Model; Animals; Artificial Insemination; Breeding; Caring; Cytomegalovirus; Derivation procedure; Development; Fostering; Future; HIV; Health; Herpesvirus 1; Housing; Human; Infant; Lymphocryptovirus; Macaca; Macaca mulatta; Maintenance; Measles; Monitor; Mothers; Partner in relationship; Pathogenesis; Population; Practice Management; Research; Resources; Rhadinovirus; Rhesus; SIV; Simian Foamy Virus; Simian Retroviruses; Testing; Time; Vaccines; Viral Vector; Virus; animal resource; base; experience; germ free condition; improved; innovation; novel; programs; social

PROJECT SUMMARY, Core A: Husbandry and Management Core Expanded SPF (eSPF) rhesus macaques (Macaca mulatta), which are defined as being negative to rhesus rhadinovirus, simian immunodeficiency virus, simian foamy virus, simian T-lymphotropic virus, simian retrovirus, macacine herpesvirus-1 (BV), measles, and rhesus cytomegalovirus, are necessary to study an increasing range of viruses important to human health, as well as the development and testing of novel viral vectors. eSPF macaques have become the premier animal model for HIV/AIDS research, including studies on HIV pathogenesis, AIDS-related opportunistic infections, and the development of HIV treatments and vaccines. Currently, the national need for genetically characterized eSPF rhesus macaques for HIV/AIDS research far exceeds the current availability. Therefore it is imperative that the available eSPF rhesus macaque populations grow in size. The successful maintenance of expanded SPF colonies is challenging, however, and requires strict management practices in order to remain specific pathogen free. With over 18 years’ experience in establishing and maintaining a colony of eSPF animals, the ONPRC is the leader at managing eSPF rhesus macaques. The overall aims of the Husbandry and Management Core are to breed and grow the ONPRC eSPF colony in order to meet existing and future animal resource demands, continue to provide and improve upon the existing exemplary eSPF husbandry and veterinary care, and to improve the eSPF resource by making the entire colony lymphocryptovirus negative. To do this we will provide innovative team based management practices including expansion of the colony into outdoor sheltered housing, introduction of a time- mated breeding program and artificial insemination, a foster mother program for abandoned infants, implementation of new enrichment and social monitoring strategies, and re-derivation of LCV negative infants from within the eSPF colony.

项目摘要，核心 A：畜牧和管理核心 扩展 SPF (eSPF) 恒河猴 (Macaca mulatta)，被定义为对恒河猴呈阴性 鼻病毒、猿猴免疫缺陷病毒、猿猴泡沫病毒、猿猴 T 淋巴细胞病毒、猿猴 逆转录病毒、马卡辛疱疹病毒-1 (BV)、麻疹病毒和恒河猴巨细胞病毒是研究 对人类健康重要的病毒种类不断增加，以及新型病毒的开发和测试 eSPF 猕猴已成为艾滋病毒/艾滋病研究的主要动物模型，包括关于艾滋病毒/艾滋病的研究。 HIV 发病机制、与 AIDS 相关的机会性感染以及 HIV 治疗方法和疫苗的开发。 目前，国家对用于艾滋病毒/艾滋病研究的基因特征 eSPF 恒河猴的需求远 因此，可用的 eSPF 恒河猴势在必行。 然而，成功维持扩大的 SPF 菌落规模具有挑战性。 并需要严格的管理实践，以保持超过 18 年的无特定病原体。 ONPRC 在建立和维持 eSPF 动物群体方面拥有丰富的经验，在管理方面处于领先地位 eSPF 恒河猴的饲养和管理核心的总体目标是培育和饲养恒河猴。 ONPRC eSPF 群体为了满足现有和未来的动物资源需求，继续提供和 改进现有的示范性 eSPF 畜牧业和兽医护理，并改善 eSPF 资源 通过使整个菌落淋巴隐病毒呈阴性，我们将提供基于创新的团队。 管理实践包括将殖民地扩展到户外庇护所、引入时间限制 交配繁殖计划和人工授精、弃婴养母计划、 实施新的丰富和社会监测策略，并重新衍生 LCV 阴性婴儿 来自 eSPF 群体内部。