Component: Viral Testing Core

组件：病毒检测核心

• 批准号: 10666557
• 负责人: Gregory A Dissen
• 金额: $ 22.7万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10666557
• 关键词: Component; Viral; Testing; Core

PROJECT SUMMARY, Core B: Viral Testing Core Genetically characterized rhesus macaques that are free of endemic viral pathogens have become the premier animal model for HIV/AIDS research, including studies on HIV pathogenesis, AIDS-related opportunistic infections, as well as the development of HIV treatments and vaccines. The number of viruses removed from specific pathogen-free (SPF) monkeys has been increasing, leading to the formation of so-called "expanded SPF" colonies. As many of these endemic simian viruses are close homologues of human viruses, expanded SPF animals are also used to study an increasing range of viruses important to human health and for the development and testing of novel viral vectors. Finally, the establishment of expanded pathogen-free colonies also serves to protect animal caretakers from zoonotic diseases and improves overall animal health. However, the successful derivation and maintenance of expanded SPF colonies is challenging and requires strict surveillance regimens for many viruses over long periods of time. Therefore, the overall aim of the Viral Testing Core is to provide appropriate pathogen surveillance and implement state-of-the-art testing services, in order to maintain our expanded SPF colony free of disease. To do this, we will use proven virus screening algorithms combined with an expanding arsenal of screening assays. A central strategy to achieve this will be the implementation of a new multiplexed protein microarray assay platform for routine colony screening, which we have recently developed and validated at our center.

项目摘要，核心 B：病毒检测核心 不含地方性病毒病原体的基因特征恒河猴已成为首选 用于艾滋病毒/艾滋病研究的动物模型，包括艾滋病毒发病机制、艾滋病相关机会性研究 感染，以及艾滋病毒治疗和疫苗的开发。删除的病毒数量 无特定病原体（SPF）的猴子不断增加，导致形成所谓的“扩大” SPF”菌落。由于许多这些地方性猿猴病毒与人类病毒密切同源，因此扩大了 SPF 动物还用于研究越来越多对人类健康和人类健康至关重要的病毒。 新型病毒载体的开发和测试。最后，建立扩大的无病原体菌落 还有助于保护动物饲养员免受人畜共患疾病的侵害并改善动物的整体健康。然而， 成功衍生和维持扩大的 SPF 菌落具有挑战性，需要严格的 对许多病毒的长期监测方案。因此，病毒检测的总体目标 核心是提供适当的病原体监测并实施最先进的检测服务，以便 保持我们扩大的 SPF 菌落免受疾病侵害。为此，我们将使用经过验证的病毒筛查算法 与不断扩大的筛选试验库相结合。实现这一目标的核心战略将是 实施新的多重蛋白质微阵列分析平台，用于常规菌落筛选，我们 最近在我们的中心开发并验证。