Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens

用于冷冻保存独特人类生物样本的液氮冷冻机自动化

• 批准号: 10533708
• 负责人: Alfredo Alberto Molinolo
• 金额: $ 29.23万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2024-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10533708
• 关键词: Accreditation; American; Automation; Basic Science; Biological Assay; California; Cancer Center; Clinic; Clinical Research; Cold Chains; Collaborations; Collection; Computer software; Cryopreservation; Database Management Systems; Ensure; Environment; Equipment; Equipment and supply inventories; Freezing; Funding; Genomics; Goals; Health; Histology; Human; Libraries; Link; Liquid substance; Manuals; Measures; Medical center; Nitrogen; Pathologist; Proteomics; Quality Control; Reporting; Reproducibility of Results; Research; Research Personnel; Resource Sharing; Retrieval; Robotics; Safety; Sampling; Security; Solid; System; Technology; Temperature; Time; Tissues; Universities; Update; biobank; college; cryogenics; improved; instrument; patient derived xenograft model; preservation; robotic system; transcriptomics

PROJECT SUMMARY/ABSTRACT This application by the Biorepository and Tissue Technology Shared Resource (BTTSR) requests funding for a BioStore™ M60 Cryo-190°C liquid nitrogen (LN 2) system to support ongoing collection of human solid biospecimen and long-term storage for future research, a goal in line with those of the NCI-designated comprehensive Moores Cancer Center (MCC), at the University of California, San Diego. This automated BioStore M60 Cryo system is a cryogenic storage unit that offers sample storage automation, cold chain management, and improved security and accessibility while maintaining sample protection within a liquid nitrogen environment. The requested instrument goes beyond conventional manual LN2 Freezers with the highest sample protection, comprehensive inventory management systems, superior user safety measures, and LIMS connectivity. The BioStore™ M60 Cryo -190°C System provides sample integrity, security and tracking with availability of Full 21-CFR-11 reporting capability. Adequate preservation of biospecimens has been proven to be critical to obtain reliable and reproducible results in genomics, transcriptomics, proteomics, developing PDX models, immunoprofiling and many other assays. Biological assays which depend on -190°C stored biospecimens can be extremely sensitive to transient temperature exposure thus the superior quality control measures of this system are needed. The new BioStore™ M60 Cryo -190°C system will ensure that our users have access to high quality human biospecimens, and the automation of the system will also enhance the integrity of samples during storage and retrieval by minimizing transient temperature exposure. The BioStore™ M60 Cryo -190°C system will be located, administered and maintained in the existing BTTSR facility at the MCC.

项目摘要/摘要 Biorepository和Tissue Technology共享资源（BTTSR）的应用要求为A要求提供资金 Biostore™M60 Cryo-190°C液氮（LN 2）系统，以支持正在进行的人类固体收集 生物测量和长期存储供未来研究，这是一个与NCI指定的目标 加州大学圣地亚哥分校的综合摩尔癌中心（MCC）。这是自动化的 Biostore M60冷冻系统是一个低温存储单元，可提供样品存储自动化，冷链 管理，并提高安全性和可访问性，同时维持液体中的样品保护 氮环境。所请求的仪器超出了传统的手册LN2冰柜， 最高样本保护，全面库存管理系统，卓越的用户安全措施以及 LIMS连接性。 BioStore™M60冷冻-190°C系统提供样本完整性，安全性和跟踪 具有完整的21-CFR-11报告能力。 事实证明，充分保存生物测量对于获得可靠且可重复的结果至关重要 在基因组学，转录组学，蛋白质组学，开发PDX模型，免疫蛋白和许多其他测定中。 依赖于-190°C的生物测定的生物测定可能对瞬态非常敏感 温度暴露因此需要该系统的优质控制措施。新的Biostore™ M60冷冻-190°C系统将确保我们的用户能够获得高质量的人类生物测量和 系统的自动化还将通过最小化在存储和检索过程中增强样品的完整性 瞬态温度暴露。 BioStore™M60冷冻-190°C系统将位于，管理和管理 维护在MCC现有的BTTSR工厂中。