Establishing Automated Cryopreservation System for Biospecimen Storage

建立生物标本储存自动化冷冻保存系统

• 批准号: 10533660
• 负责人: Camila Hochman-Mendez
• 金额: $ 22.08万
• 依托单位: TEXAS HEART INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10533660
• 关键词: Accreditation; Advertising; Agreement; American; Attention; Automation; Back; Basic Science; Biological; Biological Specimen Banks; Biomedical Research; Cardiovascular system; Clinical; Clinical Trials; Collection; Communities; Complex; Computer software; Consumption; Core Facility; Cryopreservation; Data; Development; Disasters; Disease; Emergency Situation; Ensure; Environment; Equipment; Equipment and supply inventories; Exposure to; Foundations; Freezing; Funding; Goals; Grant; Heart; High temperature of physical object; Hour; Human Resources; Infrastructure; Institutes; International; Laboratories; Liquid substance; Maintenance; Manuals; Modernization; Monoclonal Antibody R24; Nitrogen; Outcome; Pathologist; Performance; Play; Preparation; Procedures; Process; Provider; Quality Control; Reaction Time; Reproducibility; Research; Research Personnel; Research Project Grants; Resource Sharing; Retrieval; Role; Sampling; Secure; Services; Societies; Speed; Standardization; System; Technology; Temperature; Texas; Time; Training; Translational Research; United States Food and Drug Administration; base; biobank; cardiovascular health; college; cost; demographics; evidence base; improved; operation; precision medicine; preservation; repository; sample collection; specific biomarkers

Biobanking has been playing an increasingly essential role in biomedical research, growing from simple biospecimen repositories to large complex infrastructure-based systems. With the emergence of omics-based technologies and the ever-increasing demand of biospecimens, there is a recent surge in the number of biorepositories. Consequently, biorepositories are undergoing modernization by implementing state-of-the-art features of sample acquisition, quality control, storage and preservation, and analysis of disease-specific biomarkers, supplemented with well-annotated demographics and clinical/biological data, with the goal of precision medicine outcomes. The Texas Heart Institute (THI)’s College of American Pathologists-accredited and US Food and Drug Administration- and International Society for Biological and Environmental Repositories -compliant Biorepository and Biospecimen Profiling Core Laboratory (THI-BRC) has a large liquid nitrogen (LN2) cryopreservation system for long-term preservation of biospecimens. As a shared resource facility, THI-BRC is providing valuable service, such as sample (mainly cardiovascular) collection, quality-control, processing, storage and preservation, analysis, and inventory maintenance, to internal THI investigators and collaborators throughout the nation, supporting various federal/state/nongovernment-funded basic science and translational research projects and clinical trials. Given the increasing need of large numbers of high-quality samples by our investigators and collaborators, particular attention is needed for sample acquisition, preparation, preservation, retrieval, and inventory management, which can be met by installing an automated cryopreservation workstation. This project will involve installation of the BioStore Cryo III automated cryopreservation system in THI-BRC and its integration into our biorepository current practices, including the development of equipment-specific standard operating procedures (Aim 1), and training THI-BRC personnel in the use and maintenance of this automated cryopreservation system and advertising to the research community the availability of this modernized storage system with increased sample storage capabilities and quality control (Aim 2). Importantly, the proposed system does not require any changes to the current infrastructure. Automated cryopreservation will be implemented to varying degrees at various stages of our biorepository operations to improve sample access, cost, and throughput, offering both quantitative and substantial benefits to the workflow, such as integrated sample traceability, secure storage, increased retrieval speed, and sample preservation. The operation workflow in our biorepository will be standardized and controlled while reducing the time of rack exposure outside the LN2 freezer temperature. Along with mitigating temperature fluctuation of not-intended-to-be-thawed biospecimens, thereby maintaining structural integrity and functional attributes of the biospecimens, the automated cryopreservation system will overcome time-consuming manual processes, improve the efficiency of workflow, and benefit THI’s internal and external investigators’ basic and translational research initiatives on cardiovascular health.

生物群在生物医学研究中一直起着越来越重要的作用，从简单的 基于大型复杂基础设施的系统的生物测量存储库。随着基于OMIC的出现 技术和生物测量的需求不断增加，最近的数量有所激增 生物座席。因此，生物库正在实施最先进的情况下进行现代化 样品采集，质量控制，存储和制备的特征以及疾病特异性的分析 生物标志物，补充了通知的人口统计和临床/生物数据，目的是 精密医学结果。德克萨斯心脏研究所（THI）的美国病理学家学院获得了认可 以及美国食品和药物管理局和国际生物学和环境储藏库协会 - 集合生物座和生物测量分析核心实验室（Thi-BRC）具有较大的液氮（LN2） 冷冻保存系统，用于长期制备生物测量系统。作为共享资源设施，Thi-Brc是 提供价值服务，例如样本（主要是心血管）收集，质量控制，处理， 对内部调查人员和合作者的存储和准备，分析和库存维护 在全国范围内，支持各种联邦/州/非政府资助的基础科学和翻译 研究项目和临床试验。考虑到我们对大量高质量样本的需求越来越多 调查人员和合作者，需要特别关注样本获取，准备，准备， 检索和库存管理，可以通过安装自动冷冻保存工作站来满足。 该项目将涉及在Thi-BRC中安装Biostore Cryo III自动冷冻保存系统，并 它集成到我们的生物座席当前实践中，包括开发特定于设备的标准 操作程序（AIM 1），并在使用和维护此自动化方面培训Thi-BRC人员 冷冻保存系统和向研究社区广告此现代化存储的可用性 具有增加样品存储功能和质量控制的系统（AIM 2）。重要的是，拟议的系统 不需要对当前基础架构进行任何更改。自动化的冷冻保存将被实施 在我们的生物座席操作的各个阶段，各个程度的不同程度，以改善样本访问，成本和 吞吐量，为工作流提供定量和实质性好处，例如集成样本 可追溯性，安全存储，提高检索速度和样品保存。我们的操作工作流程 在减少LN2冰箱外的架子曝光时，生物座将进行标准化和控制 温度。随着降低未关注的生物测量的温度波动 维持生物测量的结构完整性和功能属性，自动冷冻保存 系统将克服耗时的手动流程，提高工作流程的效率，并使Thi的效率受益 内部和外部研究人员的基本和翻译有关心血管健康的研究计划。