Biorepository and Tissue Technology Shared Resource

生物样本库和组织技术共享资源

• 批准号: 10666380
• 负责人: Alfredo Alberto Molinolo
• 金额: $ 37.06万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10666380
• 关键词: AGTR2 gene; Academic Training; Accreditation; American; Animal Experiments; Antibodies; Bioinformatics; Biological; Biological Assay; Biomedical Research; Biopsy; Blood; Cancer Center; Cancer Center Support Grant; Cell Survival; Cellularity; Clinical; Clinical Research; Clinical Trials; Collaborations; Collection; Consent; Consultations; Core Biopsy; Cytology; Data; Dedications; Development; Diagnosis; Disease; Drug or chemical Tissue Distribution; Employment; Enrollment; Epidemiology; Equipment; Equipment and supply inventories; Excision; Experimental Designs; Experimental Pathology; Formalin; Freezing; Guidelines; Health system; Histologic; Histology; Histopathology; Hour; Human; Human Pathology; Immunofluorescence Immunologic; Immunohistochemistry; Immunophenotyping; Informed Consent; Infrastructure; Institution; Institutional Review Boards; Interview; Ischemia; Knowledge; Link; Liquid substance; Medical; Mission; Modeling; Molecular; Mus; NCI-Designated Cancer Center; Necrosis; Needles; Operative Surgical Procedures; Outcome; Paraffin; Pathologist; Pathology; Patient Admission; Patients; Peripheral Blood Mononuclear Cell; Phenotype; Physicians; Plasma; Policies; Preparation; Price; Procedures; Protocols documentation; Provider; Reaction Time; Recording of previous events; Research Personnel; Resource Sharing; Resources; Sampling; Scanning; Serum; Services; Slide; Solid; Solid Neoplasm; Specimen; Stains; Surgeon; System; Techniques; Technology; Tissue Banks; Tissue Fixation; Tissue Microarray; Tissue Preservation; Tissue Procurements; Tissue Sample; Tissues; Training; Tumor Bank; Validation; Whole Blood; anticancer research; biobank; college; cost effective; design; digital; digital imaging; experience; human tissue; member; multimodality; neoplastic cell; novel; prospective; quality assurance; tissue fixing; tissue processing; translational study; tumor

BIOREPOSITORY AND TISSUE TECHNOLOGY SHARED RESOURCE ABSTRACT The Biorepository and Tissue Technology Shared Resource (BTTSR) is a critical component of the infrastructure supporting translational studies. It is founded on a well-established institutional model integrating tissue procurement, processing, storage, and multimodal (histologic, immunohistochemical, and molecular) analyses with traditional epidemiologic, clinicopathologic and outcome parameters through a highly evolved bioinformatics system. Alfredo Molinolo, a physician pathologist with more than 40 years’ experience in human and experimental pathology, is the Director of the BTTSR. As of January 2018, the Biorepository (BRPT) is now a full College of American Pathologist (CAP)-accredited resource. Under its own IRB approved protocol (IRB# 090401), the BRPT integrates patient consenting, prospective procurement services, protocol and standard operating procedure design, tissue processing, and storage. Jason Sicklick, a GI surgeon, is the Deputy Director of the BRPT for clinical affairs. Sharmeela Kaushal is the BRPT Manager overseeing operational aspects of the BRPT under NCI and CAP guidelines. Three coordinators for patient consenting and two technicians for tissue processing assist her. Valeria Estrada, the Assistant Director for Tissue Technology, supervises the Tissue Technology Shared Resource (TTSR). She is a well-trained academic pathologist with experience in management and employment policies, and extensive knowledge of standard/novel pathology techniques. She supervises four dedicated histotechnicians. TTSR services include tissue fixation, processing and sectioning, paraffin and frozen, routine stains, special stains, individualized immunohistochemistry (IHC) and immunofluorescence (IF) assays, antibody optimization and validation, digital imaging and digital image storage, mouse phenotyping, tissue microarrays developing and construction, multiplexing IHC and IF, and pathology consultation and training, among other services. Since July 2016, the TTSR equipment inventory has grown with the acquisition of, among other equipment, an AT2 ScanScope slide scanner, a Gemini H&E stainer, and a Biocare Medical immunohistochemistry autostainer. Over the past 5 years, the BTTSR has supported 174 users, including 89 MCC members and 11 members from the Salk and Sanford-Burnham Prebys NCI-designated Cancer Centers.

生物验证和组织技术共享资源摘要 生物座和组织技术共享资源（BTTSR）是 支持翻译研究的基础设施。它建立在一个完善的机构模型上 组织采购，加工，存储和多模式（组织学，免疫组织化学和分子） 通过高度进化的传统流行病学，临床病理学和结果参数进行分析 生物信息学系统。 Alfredo Molinolo，一位具有40多年人类经验的物理病理学家 实验病理学是BTTSR的主任。截至2018年1月，生物座席（BRPT）是 现在是一名完整的美国病理学家学院（CAP）认可的资源。根据其自己的IRB批准协议 （IRB＃090401），BRPT整合了患者同意，潜在的采购服务，协议和 标准操作程序设计，组织处理和存储。胃肠道外科医生Jason Sicklick是 BRPT临床事务的副主任。 Sharmeela Kaushal是监督的BRPT经理 根据NCI和CAP指南，BRPT的操作方面。三个用于患者同意和的协调员 组织加工的两种技术有助于她。组织技术助理主管Valeria Estrada， 监督组织技术共享资源（TTSR）。她是一位训练有素的学术病理学家 具有管理和就业政策的经验，以及对标准/新型病理的广泛了解 技术。她监督了四位专门的历史学家。 TTSR服务包括组织固定，处理 以及切片，石蜡和冷冻，常规皮肤，特殊皮肤，个性化免疫组织化学（IHC） 和免疫荧光（如果）测定，抗体优化和验证，数字成像和数字图像 存储，小鼠表型，组织微阵列的发展和构造，多重IHC以及IF，以及 病理咨询和培训以及其他服务。自2016年7月以来，TTSR设备库存 随着AT2 Scanscope幻灯片扫描仪的收购，GEMINI H＆E成长 染色器和生物保健医学免疫组织化学自动化器。在过去的5年中，BTTSR有 支持174位用户，包括来自Salk和Sanford-Burnham的89名MCC成员和11名成员 Prebys NCI指定的癌症中心。